The F685/F730 chlorophyll fluorescence ratio as indicator of chilling stress in plants
The F685/F730 chlorophyll fluorescence ratio as indicator of chilling stress in plants
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DOI：
10.1016/s0176-1617(96)80270-7
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发表时间：
1996-05-01
影响因子：
4.3
通讯作者：
Cecchi, G
中科院分区：
文献类型：
--
作者：
Agati, G;Mazzinghi, P;Cecchi, G
通过两个不同的实验评价了叶绿素荧光对低温的响应。首先，测定了菜豆F685/F730和F-v/F-m的叶绿素荧光比值。蒙脱莲植株在4℃的低温胁迫和中等光照(100Mmolm(-2)S(-1))下持续72小时。F-v/F-m随冷藏时间呈线性下降，表明有光抑制效应(在相同条件下黑暗条件下观察不到变化)。F685/F730细胞经历了快速指数衰减和线性缓慢下降。在第二个实验中，在气候室中，当温度从20℃降低到4℃时，监测了单叶上的F685/F730比值、总叶绿素荧光、F685+F730和叶温。穿梭)植物。对于这两个物种，与叶温有关的两个阶段可以被区分出来：第一个4小时期间，叶温从24℃下降到4℃，第二个阶段，叶温在4℃左右略有波动。在第一阶段，对低温敏感的豌豆的抗寒性显著降低，而对抗冷性较强的豌豆的抗冷性略有增加。在接下来的一段时间里，豌豆的F685/F730值保持不变，而豆类的F685/F730值仍然下降。根据我们的结果，可以考虑使用叶绿素荧光比率作为植物冷害敏感度的指示器。
The response of chlorophyll fluorescence to chilling temperatures was evaluated by two different experiments. In the first, the F685/F730 and the F-v/F-m chlorophyll fluorescence ratios were measured in Phaseolus vulgaris L., cv. Mondragone plants under chilling stress at 4 degrees C and moderate light (100 mu mol m(-2) s(-1)) up to 72 hours. F-v/F-m decreased linearly with chilling time indicating a photoinhibitory effect (no change was observed in the dark under the same conditions). F685/F730 underwent a rapid exponential decay followed by a linear slow decline. In a second experiment, the F685/F730 ratio, the total chlorophyll fluorescence, F685 + F730, and the leaf temperature were monitored on a single leaf in a climate chamber as the temperature was decreased from 20 to 4 degrees C. The experiment was run simultaneously on the chilling-sensitive Phaseolus vulgaris and on the chilling-tolerant Pisum sativum L. (cv. Shuttle) plants. For both species two phases related to the leaf temperature can be distinguished: the first 4-hour period during which the leaf temperature decreased from 24 to 4 degrees C, and a second period during which the leaf temperature slightly oscillated around 4 degrees C. The behaviour of F685/F730 for the bean was quite different from that of the pea plant. During the first phase, it decreased markedly for the chilling-sensitive bean while a slight increase was observed for the chilling-resistant pea. In the following period, the F685/F730 values for the pea remained constant while those for the bean were found still to decrease. On the basis of our results, the use of the chlorophyll fluorescence ratio as indicator of plant chilling sensitivity can be envisaged.