Isolation and characterization of light-regulated phycobilisome linker polypeptide genes and their transcription as a polycistronic mRNA.
Isolation and characterization of light-regulated phycobilisome linker polypeptide genes and their transcription as a polycistronic mRNA.
复制标题
光调节藻胆体接头多肽基因的分离和表征及其作为多顺反子 mRNA 的转录。
DOI：
10.1128/jb.169.6.2675-2684.1987
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发表时间：
1987
影响因子：
3.2
通讯作者：
Grossman,AR
中科院分区：
文献类型：
--
作者：
Lomax,TL;Conley,PB;Schilling,J;Grossman,AR
几种蓝细菌调整藻胆体的藻胆蛋白和连接蛋白的组成，藻胆体是蓝细菌和一些真核藻类中的捕光复合物，以最大限度地吸收普遍波长的光。这个过程被称为互补色适应。我们测序的连接多肽的氨基末端，这是与藻蓝蛋白和积累到高水平的蓝细菌Fremyella diplosiphon在红光下生长。合成了编码该氨基末端区域的混合寡核苷酸，并用于鉴定F.在一些实施方案中，所述连接子多肽包含编码连接子多肽的倍管藻基因组DNA。该连接基因位于另外两个连接基因之间，并且与红光诱导的藻蓝蛋白基因组相邻。提供了所有三个接头基因的序列。这些基因一起转录到一个大的多顺反子mRNA，它也编码红光诱导的藻蓝蛋白亚基。该转录本与藻胆体生物发生的关系。讨论了在不同光照条件下生长双倍管藻的条件。
Several cyanobacteria adjust both the phycobiliprotein and linker protein composition of the phycobilisome, a light-harvesting complex in cyanobacteria and some eucaryotic algae, to maximize absorption of prevalent wavelengths of light. This process is called complementary chromatic adaptation. We sequenced the amino terminus of a linker polypeptide which is associated with phycocyanin and accumulates to high levels during growth of the cyanobacterium Fremyella diplosiphon in red light. A mixed oligonucleotide encoding a region of this amino terminus was synthesized and used to identify a fragment of F. diplosiphon genomic DNA encoding the linker polypeptide. This linker gene was located between two other linker genes and contiguous to the red-light-induced phycocyanin gene set. Sequences of all three linker genes are presented. These genes were transcribed together onto a large polycistronic mRNA which also encoded the red-light-induced phycocyanin subunits. The relationship of this transcript to the biogenesis of the phycobilisome when F. diplosiphon is grown under different conditions of illumination is discussed.