Two Figla homologues have disparate functions during sex differentiation in half-smooth tongue sole (Cynoglossus semilaevis).
Two Figla homologues have disparate functions during sex differentiation in half-smooth tongue sole (Cynoglossus semilaevis).
复制标题
两个 Figla 同源物在半滑舌鳎 (Cynoglossus semilaevis) 性别分化过程中具有不同的功能
DOI：
10.1038/srep28219
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发表时间：
2016-06-17
影响因子：
4.6
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Li H;Xu W;Zhang N;Shao C;Zhu Y;Dong Z;Wang N;Jia X;Xu H;Chen S
Figla is a germ-cell-specific transcription factor associated with ovary development and differentiation. In vertebrates, one transcriptional form of Figla is commonly found. However, besides the common form of this gene (named Figla_tv1), a new transcriptional form (named Figla_tv2) was identified in half-smooth tongue sole (Cynoglossus semilaevis). The full-length cDNA of Figla_tv1 was 1057 bp long with a 591-bp open reading frame encoding a predicted 196 amino acid protein, while Figla_tv2 encoded a 125 amino acid protein. Figla_tv1 and Figla_tv2 expression in various tissues was detected by qRT-PCR. Figla_tv1 was expressed mainly in ovary, skin and liver, while Figla_tv2 was expressed in all examined tissues. In the gonads, Figla_tv1 was expressed in ovary, while Figla_tv2 was predominately expressed in testis of pseudomales. Further, in situ hybridization located Figla_tv1 only in oocytes and Figla_tv2 mainly in germ cells of pseudomale testis. After knocking down Figla_tv2 in a pseudomale testis cell line, the expression of two steroid hormone-encoding genes, StAR and P450scc, was significantly up-regulated (P 0.05). Our findings suggest that Figla_tv1 has a conserved function in folliculogenesis, as in other vertebrates, and that Figla_tv2 may have a role in the spermatogenesis of pseudomales by regulating the synthesis and metabolism of steroid hormones.
