Epidermal cytokines IL-1β, TNF-α, and IL-12 in patients with atopic dermatitis:: Response to application of house dust mite antigens
Epidermal cytokines IL-1β, TNF-α, and IL-12 in patients with atopic dermatitis:: Response to application of house dust mite antigens
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DOI：
10.1046/j.1523-1747.1998.00409.x
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发表时间：
1998-12-01
影响因子：
6.5
通讯作者：
Neumann, C
中科院分区：
文献类型：
--
作者：
Junghans, V;Gutgesell, C;Neumann, C
表皮细胞因子，如白细胞介素（IL）-1 β，肿瘤坏死因子-α，和IL-12已被描述为在过敏性接触性皮炎的诱导和激发阶段暴露于半抗原后发挥关键作用，在这项研究中，我们问这些细胞因子是否也可能发挥作用，在过敏性皮炎患者的表皮后，应用屋尘螨抗原（HDM）到他们的皮肤。在施用HDM提取物后8小时，通过刮擦特应性患者和健康个体的健康外观皮肤来收集表皮样品。十二烷基硫酸钠和生理盐水作为对照。对IL-1 β、肿瘤坏死因子-α、IL-12 p35和IL-12 p40进行逆转录酶-聚合酶链反应。暴露于HDM导致仅在特应性患者中这些细胞因子的mRNA显著上调。而IL-1 β和肿瘤坏死因子-α也显示了在暴露于刺激性十二烷基硫酸钠后，在这些患者的一部分上调，IL-12 p40 mRNA的应用过敏原的专门增强，与IL-12 p40相反，IL-12 p35 mRNA检测不到显着的量。有趣的是，在未经治疗的、外观正常的特应性个体（n = 16）皮肤中，这些细胞因子的水平也高于正常个体（n = 8），这可能解释了特应性个体皮肤刺激性的增加。最后，比较过敏原斑贴试验阳性患者与阴性患者皮肤中的表皮细胞因子，表明只有IL-1 β mRNA的表达可能是对HDM的阳性斑贴试验反应的预测标志物。
Epidermal cytokines such as interleukin (IL)-1 beta, tumor necrosis factor-alpha, and IL-12 have been described to play a crucial role in the induction and elicitation phase of allergic contact dermatitis upon exposure to haptens, In this study we asked whether these cytokines may also play a role in the epidermis of patients with atopic dermatitis after the application of house dust mite antigens (HDM) to their skin. Epidermal samples were collected by scraping healthy appearing skin of atopic patients and healthy individuals 8 h after the application of an extract of HDM. Sodium lauryl sulfate and saline served as controls. Reverse transcriptase-polymerase chain reaction was performed for IL-1 beta, tumor necrosis factor-alpha, IL-12 p35, and IL-12 p40. Exposure to HDM led to a significant upregulation of mRNA of these cytokines in atopic patients only. Whereas IL-1 beta and tumor necrosis factor-alpha also showed an upregulation in part of these patients after exposure to the irritant sodium lauryl sulfate, IL-12 p40 mRNA was exclusively enhanced by the application of the allergen, In contrast to IL-12 p40, IL-12 p35 mRNA was not detectable in significant amounts. Interestingly, also in untreated, normal appearing skin of atopic individuals (n = 16), the levels of these cytokines were higher than in normal individuals (n = 8), possibly explaining the increased skin irritability of atopic individuals. Finally, comparing epidermal cytokines in the skin of patients who developed a positive allergen patch test to those who stayed negative, suggests that only expression of IL-1 beta mRNA may be a predictive marker for the development of a positive patch test reaction to HDM.