Reduced accumulation of specific microRNAs in colorectal neoplasia.
Reduced accumulation of specific microRNAs in colorectal neoplasia.
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发表时间：
2003-10
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通讯作者：
M. Michael;Susan M O' Connor;Nicholas G van Holst Pellekaan;G. Young;R. James
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作者：
M. Michael;Susan M O' Connor;Nicholas G van Holst Pellekaan;G. Young;R. James
已知短的非编码RNA调节细胞过程，包括真核生物中的发育、异染色质形成和基因组稳定性。考虑到这些过程对细胞特性的影响，进行了一项研究以调查肿瘤发生期间microRNA（miRNA）水平的可能变化。在结肠腺癌和正常粘膜中总共鉴定了28种不同的miRNA序列，包括3种新序列和另外7种先前仅从小鼠中克隆的序列。小鼠miRNA序列的人类同源物miR-143和miR-145在结直肠瘤形成的腺瘤和癌症阶段一致地显示成熟miRNA的稳态水平降低。
Short non-coding RNAs are known to regulate cellular processes including development, heterochromatin formation, and genomic stability in eukaryotes. Given the impact of these processes on cellular identity, a study was undertaken to investigate possible changes in microRNA (miRNA) levels during tumorigenesis. A total of 28 different miRNA sequences was identified in a colonic adenocarcinoma and normal mucosa, including 3 novel sequences and a further 7 that had previously been cloned only from mice. Human homologues of murine miRNA sequences, miR-143 and miR-145, consistently display reduced steady-state levels of the mature miRNA at the adenomatous and cancer stages of colorectal neoplasia.