High-density lipoprotein subfractions as measured by differential polyanionic precipitation and rate zonal ultracentrifugation.
High-density lipoprotein subfractions as measured by differential polyanionic precipitation and rate zonal ultracentrifugation.
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通过微分聚阴离子沉淀和速率区带超速离心测量高密度脂蛋白亚组分。
DOI：
10.1093/clinchem/28.10.2040
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发表时间：
1982
影响因子：
9.3
通讯作者：
J. Shepherd
中科院分区：
文献类型：
--
作者：
H. Simpson;F. Ballantyne;C. Packard;H. G. Morgan;J. Shepherd
最近，人们对高密度脂蛋白(HDL)及其亚组分对动脉粥样硬化的保护作用感兴趣，这突显了快速、简单的亚组分程序的必要性。在这里，我们比较了最近发展的两种多阴离子沉淀法与速率区带超速离心法分离高密度脂蛋白的结果。三种方法对总高密度脂蛋白胆固醇的测定结果相似。然而，沉淀法测得的HDL2胆固醇显著高于地带值，而HDL3则较低。这反映了分离所涉及的不同基本原则，并突出表明需要更清楚地了解高密度脂蛋白组分的功能作用。
Recent interest in the putative protective role of high-density lipoprotein (HDL) and its subfractions against atherosclerosis has highlighted the need for a rapid, simple subfractionation procedure. Here we compared HDL subfractionation by two recently developed polyanionic-precipitation methods with the values obtained by rate zonal ultracentrifugation. A similar result for total HDL cholesterol was obtained by all three methods. However, HDL2 cholesterol as measured by the precipitation procedures was significantly higher than the zonal value, and HDL3 was lower. This reflects the different underlying principles involved in the separations and highlights the need for a clearer understanding of the functional roles of the HDL fractions.