Differential expression profiles of sense and antisense transcripts between HCV-associated hepatocellular carcinoma and corresponding non-cancerous liver tissue
Differential expression profiles of sense and antisense transcripts between HCV-associated hepatocellular carcinoma and corresponding non-cancerous liver tissue
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DOI：
10.3892/ijo.2012.1382
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发表时间：
2012-06-01
影响因子：
5.2
通讯作者：
Ohkohchi, Nobuhiro
中科院分区：
文献类型：
--
作者：
Nagai, Kentaro;Kohno, Keisuke;Ohkohchi, Nobuhiro
近年来的研究表明，天然反义转录物是信使RNA的互补序列，具有重要的细胞功能，如mRNA的稳定和沉默。然而，尚未描述反义转录本在肝细胞癌（HCC）发展中可能的贡献。因此，我们同时研究了肝细胞癌和非癌组织的正义和反义转录本，以探讨反义转录本对肝细胞癌进展的可能贡献。从15个HCV相关的HCC和6个相应的非癌组织中制备RNA，并使用人类定制微阵列对正义和反义转录物进行表达谱分析。在肝癌和非癌组织中，有161个正义转录本和25个反义转录本的表达差异超过2倍（p
Recent studies have demonstrated that natural antisense transcripts, which are complementary sequences to messenger RNA, have important cellular functions such as the stabilization and silencing of mRNA. However, the possible contribution of antisense transcripts in hepatocellular carcinoma (HCC) development has not been described. Therefore, we simultaneously investigated the sense and antisense transcripts of HCC and non-cancerous tissues to explore the possible contribution of antisense transcripts to HCC progression. RNA was prepared from 15 HCV-associated HCCs and from 6 corresponding non-cancerous tissues and was subjected to expression profile analysis of sense and antisense transcripts using a human custom microarray. Differential expression of 161 sense and 25 antisense transcripts was observed with more than 2-fold between HCC and non-cancerous tissue (p