An in vitro Method for Making Repeated pH Measurements on Human Dental Plaque
An in vitro Method for Making Repeated pH Measurements on Human Dental Plaque
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一种对人体牙菌斑进行重复 pH 测量的体外方法
DOI：
10.1177/00220345840630062001
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发表时间：
1984
影响因子：
7.6
通讯作者：
A. Krobicka
中科院分区：
文献类型：
--
作者：
B. G. Bibby;A. Krobicka
将一种组合pH微电极插入人体斑块中，将其离心到一个紧密配合的细网丝“篮子”的底部，用于连续记录暴露于各种碳水化合物底物所产生的pH反应。持续浸泡在糖和淀粉溶液中的pH值下降，在深度和持续时间上，与通过遥测pH值测量近似斑块样品得到的结果相似。相反，短暂接触糖溶液会产生斯蒂芬型pH曲线。pH值的测量可以使用一系列不同的底物，如果在每个底物测试后，电极-斑块组装用水清洗，其酸度在循环唾液中中和。有人建议，这种体外pH值方法可以用来代替近似斑块的遥测测量，以比较从食物中产生的酸或用于其他目的。
A combination pH micro-electrode inserted into human plaque centrifuged to the base of a closely-fitting fine-mesh wire "basket" has been used to make continuous recordings of the pH responses produced by exposure to various carbohydrate substrates. The pH depressions given by continued immersion in sugar and starch solutions resemble, both in depth and duration, those obtained by telemetric pH measurements of approximal plaque samples. In contrast, brief exposure to sugar solutions will produce a Stephan type of pH curve. Measurements of pH can be made using a succession of different substrates if, after each substrate is tested, the electrode-plaque assembly is washed with water and its acidity neutralized in circulating saliva. It is suggested that this in vitro pH method could be used instead of the telemetric measurements of approximal plaque for comparing acid production from foods or for other purposes.