A possible role of histone-like DNA-binding protein of Streptococcus intermedius in the pathogenesis of bile duct damage in primary biliary cirrhosis
A possible role of histone-like DNA-binding protein of Streptococcus intermedius in the pathogenesis of bile duct damage in primary biliary cirrhosis
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DOI：
10.1016/j.clim.2008.01.010
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发表时间：
2008-05-01
影响因子：
8.6
通讯作者：
Shiratori, Keiko
中科院分区：
文献类型：
--
作者：
Haruta, Ikuko;Kikuchi, Ken;Shiratori, Keiko
细菌感染在原发性胆汁性肝硬化（PBC）的发病机制中已成为人们关注的焦点。有文献报道PBC中存在抗组蛋白自身抗体，提示细菌[组蛋白样DNA结合蛋白（HLP）]可能参与PBC的发病。为了鉴定PBC中的细菌种类以证实这种可能性，通过ELISA研究了血清对细菌细胞的反应性。PBC患者IgM类中间链球菌滴度显著高于慢性丙型肝炎病毒（CH-C）所致肝炎和健康受试者。在链球菌中，S.选择中间型进行进一步研究。S.合成HLP的中间体以检查血清对Si-HLP的反应性。IgM类抗Si-HLP肽滴度在PBC中显著更高。PBC患者肝内胆管上皮细胞和汇管区炎性细胞胞浆内均可见抗Si-HLP抗体。链球菌，尤其是S.中间肌可能在PBC的发病机制中起关键作用，可能涉及到神经元（C）2008 Elsevier Inc. All rights reserved.
Bacterial infection has become a focus of attention in the pathogenesis of primary biliary cirrhosis (PBC). It was reported that anti-histone autoantibody was detected in PBC, suggesting that bacteria[ histone-Like DNA-binding protein (HLP) may be involved in the pathogenesis of PBC. To identify bacterial, species in PBC to confirm this possibility, serum reactivity to bacterial cells was studied by ELISA. The IgM class Streptococcus intermedius titers were significantly higher in PBC than chronic hepatitis due to hepatitis C virus (CH-C) and healthy subjects. Among the streptococci, S. intermedius was selected for further study. The antigenic peptide of S. intermedius of HLP was synthesized to examine the serum reactivity to Si-HLP. IgM class anti-Si-HLP peptide titers were significantly higher in PBC. Immunoreactivity to anti-Si-HLP was detected in the cytoplasm of biliary epithelial cells and inflammatory cells in the portal area in PBC patients' livers. Streptococci, especially S. intermedius, might play a key role in the pathogenesis of PBC, possibly involving HLR (C) 2008 Elsevier Inc. All rights reserved.