Chlorogenic acid is poorly absorbed, independently of the food matrix:: A Caco-2 cells and rat chronic absorption study
Chlorogenic acid is poorly absorbed, independently of the food matrix:: A Caco-2 cells and rat chronic absorption study
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DOI：
10.1002/mnfr.200600034
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发表时间：
2006-11-01
影响因子：
5.2
通讯作者：
Maillard, Marie-Noelle
中科院分区：
文献类型：
--
作者：
Dupas, Coralie;Baglieri, Agnes Marsset;Maillard, Marie-Noelle
流行病学研究表明，膳食酚类抗氧化剂，如绿原酸（CQA），可以预防冠心病和某些癌症，咖啡是人类膳食中CQA的主要来源。本研究的目的是评估通常的咖啡消费条件，如添加牛奶，对CQA生物利用度的影响。CQA和牛奶蛋白质之间的相互作用，使用超滤技术。事实证明，这些相互作用在体外消化过程中会被轻微破坏。然后使用Caco-2细胞模型结合体外消化过程在体外研究CQA吸收和生物利用度，并在体内进行慢性补充研究，其中大鼠每天喂食咖啡或咖啡和牛奶，持续3周。这两个实验都表明，CQA在其天然形式下的吸收较弱，但未被添加乳蛋白修饰，并且通过添加美拉德反应产物略微降低。这些数据表明。在咖啡酚类物质和乳蛋白之间存在一些相互作用，但这些对大鼠中咖啡的CQA生物利用度没有显著影响。CQA在其天然形式下在体内吸收很差，当在现实的食物基质中摄入时。
According to epidemiologic studies, dietary phenolic antioxidants, such as chlorogenic acid (CQA), could prevent coronary heart diseases and some cancers, Coffee is the main source of CQA in the human diet. The aim of this study was to assess the effect of usual coffee consumption conditions, such as the addition of milk, on CQA bioavailability. Interactions between CQA and milk proteins were shown, using an ultrafiltration technique. These interactions proved to be slightly disrupted during an in vitro digestion process. CQA absorption and bioavailability were then studied in vitro using a Caco-2 cell model coupled with an in vitro digestion process, and in vivo, in a chronic supplementation study in which rats were fed daily coffee or coffee and milk for 3 weeks. Both experiments showed that CQA absorption under its native form is weak, but unmodified by the addition of milk proteins, and slightly reduced by the addition of Maillard reaction products. These data show that. there are some interactions between coffee phenolics and milk proteins, but these have no significant effect on CQA bioavailability from coffee in the rat. CQA is poorly absorbed under its native form in the body, when ingested in a realistic food matrix.