Luminescence of peptide‐bound terbium ions Determination of binding constants
Luminescence of peptide‐bound terbium ions Determination of binding constants
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肽结合铽离子的发光 结合常数的测定
DOI：
10.1016/0014-5793(91)80464-e
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发表时间：
1991
期刊：
影响因子：
3.5
通讯作者：
Andrzej Bierzyński
中科院分区：
文献类型：
--
作者：
M. Dadlez;J. Góral;Andrzej Bierzyński
研究了Tb ~（3+）离子与钙调素片段结合的发光。结果表明，在其寿命期间，激发离子从肽解离。如果游离肽的浓度足够高，它们可以再次配位。因此，观察到的铽发光寿命和强度不仅取决于结合平衡，而且还取决于浓度或游离肽分子。在这样的系统中，铽的结合常数不能通过简单的稳态发光强度测量来正确地确定。相反，必须分析在各种肽浓度下测量的铽发光衰减曲线。这样的分析已经取得了大鼠睾丸钙调素的IIIrd钙结合结构域的片段。确定了理论函数与实验点的最佳拟合所对应的速率常数或铽结合常数和平衡结合常数。
Luminescence of Tb3+ions bound to a calmodulin fragments has been studied. It is shown that during their lifetime excited ions dissociate from the peptide. If concentration of free peptide is high enough they can be coordinated again. As a consequence, observed terbium luminescence lifetime and intensity depends not only on binding equilibrium, but also on concentration or free peptide molecules. In such a system terbium binding constant cannot be correctly determined by simple steady-state measurements of luminescence intensities. Instead, terbium luminescence decay curves measured at various peptide concentrations must be analysed. Such an analysis has been made for a fragment of the IIIrd calcium binding domain of rat testis calmodulin. Rate constant or terbium association and the equilibrium binding constant corresponding to the best fit of theoretical functions to experimental points have been determined.
骨骼肌钙蛋白 C 低亲和力钙结合位点的合成肽类似物的结构和生物学研究。
DOI：
10.1016/0167-4838(87)90011-2
发表时间：
1987
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Malik,NA;Anantharamaiah,GM;Gawish,A;Cheung,HC
通讯作者：
Cheung,HC
骨骼肌钙蛋白 C 的合成肽类似物：低亲和力钙结合位点 II 类似物的荧光研究。
影响因子：
3.9
作者：
Kanellis,P;Yang,J;Cheung,HC;Lenkinski,RE
通讯作者：
Lenkinski,RE