Hepatocyte and Kupffer cells co-cultured on micropatterned surfaces to optimize hepatocyte function
Hepatocyte and Kupffer cells co-cultured on micropatterned surfaces to optimize hepatocyte function
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DOI：
10.1089/ten.2006.12.751
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发表时间：
2006-04-01
影响因子：
--
通讯作者：
Coger, Robin N.
中科院分区：
文献类型：
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作者：
Zinchenko, Yekaterina S.;Schrum, Laura W.;Coger, Robin N.
暂时延长肝功能衰竭患者生命的一种策略是使用生物人工肝（BAL）支持装置。BALs的功能成分是被称为肝细胞的实质肝细胞。进一步提高当前BAL性能水平的一种设计选择是在设计中包括肝脏的非实质细胞（例如，Kupffer细胞）。在目前的研究中，Kupffer细胞对肝细胞功能的影响通过这两种肝细胞群的微模式共培养来研究。使用传统的共培养方法，用户无法控制一种细胞类型与另一种细胞类型的相对接近度。在这项研究中，使用了两种不同的微模式技术来设计宏微模式和精细微模式配置，以评估肝细胞-库普弗细胞共培养。还调整了一个细胞群与另一个细胞群的比例，以评估对肝细胞功能的影响。微图案共培养维持10天，以评估形态和功能（如白蛋白，尿素）的变化。结果表明，微模式肝细胞，在本研究的安排，显著改善肝细胞的功能。
One strategy for temporarily extending the lives of patients with liver failure is the use of bioartificial liver (BAL) support devices. The functional components of BALs are the parenchymal liver cells known as hepatocytes. One design option for further improving current BAL performance levels is to include the non-parenchymal cells of the liver (e. g., Kupffer cells) in the design. In the current study, the effect of Kupffer cells on hepatocyte function was investigated using micropatterned co-cultures of these two liver cell populations. With traditional co-culture methods, the user is unable to control the relative proximity of one cell type to another. In this study, two different micropatterning techniques were used to engineer macro and fine micropatterned configurations for evaluating hepatocyte-Kupffer cell co-cultures. The ratio of one cell population to the other was also adjusted to evaluate the effects on hepatocyte function. The micropatterned co-cultures were maintained for ten days to evaluate for morphological and functional (e. g., albumin, urea) changes. The results illustrate that micropatterning hepatocytes, in the arrangements of this study, significantly improved hepatocyte function.