Structure and Epigenetic Regulation of Chromatin Fibers.
Structure and Epigenetic Regulation of Chromatin Fibers.
复制标题
DOI：
10.1101/sqb.2017.82.033795
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Ping-Yen Chen;Guohong Li
中科院分区：
文献类型：
--
作者：
Ping-Yen Chen;Guohong Li
在真核生物中，基因组DNA被组蛋白分层包装成染色质的几个层次，以适应细胞核内。作为核小体阵列和高级染色质组织之间的中央水平结构，30纳米染色质纤维及其动力学在基因调控中发挥着至关重要的作用。然而，尽管在过去的三十年里，相当大的努力，染色质纤维的基本结构及其动态调控仍然是一个巨大的挑战，在分子生物学。在这里，我们主要总结了最新的进展，阐明了30 nm的染色质纤维的结构在体外和染色质因子，特别是组蛋白变体的染色质纤维的表观遗传调控。此外，我们还讨论了最近的研究解开三维组织的染色质纤维原位基因组方法和电子显微镜。
In eukaryotes, genomic DNA is hierarchically packaged by histones into chromatin on several levels to fit inside the nucleus. As a central-level structure between nucleosomal arrays and higher-order chromatin organizations, the 30-nm chromatin fiber and its dynamics play a crucial role in gene regulation. However, despite considerable efforts over the past three decades, the fundamental structure and its dynamic regulation of chromatin fibers still remain as a big challenge in molecular biology. Here, we mainly summarize the most recent progress in elucidating the structure of the 30-nm chromatin fiber in vitro and epigenetic regulation of chromatin fibers by chromatin factors, particularly histone variants. In addition, we also discuss recent studies in unraveling the three-dimensional organization of chromatin fibers in situ by genomic approaches and electron microscopy.