Complete sequence and structure of the gene for human adenosine deaminase.
Complete sequence and structure of the gene for human adenosine deaminase.
复制标题
人腺苷脱氨酶基因的完整序列和结构。
DOI：
10.1021/bi00373a017
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发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
Hutton,JJ
中科院分区：
文献类型：
--
作者：
Wiginton,DA;Kaplan,DJ;States,JC;Akeson,AL;Perme,CM;Bilyk,IJ;Vaughn,AJ;Lattier,DL;Hutton,JJ
摘要：测定了人腺苷脱氨酶基因的核苷酸序列。该基因是从一系列包含人类生殖系DNA的重叠噬菌体克隆中分离出来的。共测序36741对碱基对，其中转录起始位点至多聚腺苷化位点32040对碱基对，5′侧DNA 3935对碱基对，3′侧DNA 766对碱基对。该基因包含12个外显子，由11个内含子隔开。外显子的大小为62 ~ 325个碱基对，内含子的大小为76 ~ 15 ~ 166个碱基对。该区域测序包含23个Alu重复DNA拷贝和一个O家族重复拷贝。这些重复序列除一个外都位于前三个内含子或S'侧区。该基因的表观启动子区域缺乏真核生物启动子中常见的“TATA”和“CAAT”序列，并且G/C非常丰富。该区域包含与其他富含G/ c启动子同源的区域，包括6个与转录因子Spl功能结合位点序列高度同源的十核核苷酸序列。腺苷脱氨酶（ec3.5）4.4)是嘌呤分解代谢途径的一部分，催化腺苷和脱氧腺苷的不可逆脱氨。
Revised Manuscript Received August 19, 1986 abstract: The nucleotide sequence of the human adenosine deaminase gene was determined. The gene was isolated in a series of overlapping phage clones containing human germ line DNA. A total of 36 741 base pairs were sequenced, including 32040 base pairs from the transcription initiation site to the poly-adenylation site, 3935 base pairs of 5'-flanking DNA, and 766 base pairs of 3'-flanking DNA. The gene contains 12 exons separated by 11 introns. The exons range in size from 62 to 325 base pairs while the introns are 76-15 166 base pairs in size. The area sequenced contains 23 copies of Alu repetitive DNA and a single copy of an “O” family repeat. All but one of these repeat sequences are located in the first three introns or the S'-flanking region. The apparent promoter region of the gene lacks the “TATA” and “CAAT” sequences often found in eucaryotic promoters and is extremely G/C rich. Contained within this region are areas homologous to other G/C-rich promoters, including six decanucleotide sequences that are highly homologous to sequences identified as functional binding sites for transcription factor Spl..^^. denosine deaminase (adenosine aminohydrolase, EC 3.5. 4.4), a part of the purine catabolic pathway, catalyzes the irreversible deamination of adenosine and deoxyadenosine.