Degradation of 4-nitrophenol by the lignin-degrading basidiomycete Phanerochaete chrysosporium
Degradation of 4-nitrophenol by the lignin-degrading basidiomycete Phanerochaete chrysosporium
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DOI：
10.1007/s00253-004-1637-z
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发表时间：
2004-09
影响因子：
5
通讯作者：
H. Teramoto;Hiroo Tanaka;H. Wariishi
中科院分区：
文献类型：
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作者：
H. Teramoto;Hiroo Tanaka;H. Wariishi
利用木质素降解菌黄孢原毛革菌研究了4-硝基苯酚（4-NP）的真菌代谢。尽管它的酚类特征，4-NP不被细胞外木质素分解过氧化物酶氧化。然而，4-NP转化为1，2-二甲氧基-4-硝基苯通过中间体形成4-硝基苯甲醚的真菌只有在木质素降解条件下。代谢通过芳环的羟基化和酚羟基的甲基化进行。虽然硝基还原酶参与了包括P. chrysosporium的4-NP代谢过程中没有观察到4-氨基苯酚的生成。细胞色素P450抑制剂胡椒基丁醚能有效地抑制1，2-二甲氧基-4-硝基苯的生成，表明细胞色素P450参与了羟化反应。因此，P. chrysosporium似乎利用羟基化和甲基化反应来产生更易受氧化代谢系统影响的结构。
The fungal metabolism of 4-nitrophenol (4-NP) was investigated using the lignin-degrading basidiomycete,Phanerochaete chrysosporium. Despite its phenolic feature, 4-NP was not oxidized by extracellular ligninolytic peroxidases. However, 4-NP was converted to 1,2-dimethoxy-4-nitrobenzene via intermediate formation of 4-nitroanisole by the fungus only under ligninolytic conditions. The metabolism proceeded via hydroxylation of the aromatic ring and methylation of phenolic hydroxyl groups. Although the involvement of nitroreductase in the metabolism of 2,4-dinitrotoluene by many aerobic and anaerobic microorganisms includingP. chrysosporiumhas been reported, no formation of 4-aminophenol was observed during 4-NP metabolism. The formation of 1,2-dimethoxy-4-nitrobenzene was effectively inhibited by exogenously added piperonyl butoxide, a cytochrome P450 inhibitor, suggesting that cytochrome P450 is involved in the hydroxylation reaction. Thus,P. chrysosporiumseems to utilize hydroxylation and methylation reactions to produce a more susceptible structure for an oxidative metabolic system.