Basic verification of cancer cell separation characteristics in a dielectrophoretic device using microcylindrical electrodes
Basic verification of cancer cell separation characteristics in a dielectrophoretic device using microcylindrical electrodes
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DOI：
10.1002/ecj.12328
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发表时间：
2021-10
影响因子：
0.3
通讯作者：
Hideo Miyamukai;I. Yagi;S. Uchida;Masayo Takano;Y. Wakizaka;Takaharu Enjoji
中科院分区：
文献类型：
--
作者：
Hideo Miyamukai;I. Yagi;S. Uchida;Masayo Takano;Y. Wakizaka;Takaharu Enjoji
血液中循环肿瘤细胞（CTC）的高度准确分离对于癌症的早期检测至关重要。特别是，具有三维（3D）结构的介电泳装置可以期望具有传统二维形状无法获得的分离精度。在这项研究中，分离白血病衍生的悬浮Jurkat细胞和荧光聚苯乙烯颗粒，它们是均匀大小的颗粒，使用三维微柱电极进行。当Jurkat细胞浓度降至聚苯乙烯颗粒浓度的1/100时，Jurkat细胞和聚苯乙烯颗粒可以有效分离，靶细胞收集出口II的收集效率分别为100%和2%。这表明，所提出的设备可能能够分离类似大小的细胞具有较高的精度。
Highly accurate isolation of circulating tumor cells (CTC) in the blood is essential for early detection of cancer. In particular, the dielectrophoretic device with a three‐dimensional (3D) structure can be expected to have separation accuracy, which cannot be obtained with conventional 2D shapes. In this study, an isolating leukemia‐derived suspended Jurkat cell and fluorescent polystyrene particle, which are particles of uniform size, using a 3D microcylindrical electrodes are conducted. When the concentration of Jurkat cells was reduced to 1/100 of the concentration of polystyrene particles, Jurkat cells and polystyrene particles could be efficiently separated, and the collection efficiency at Outlet II, the outlet for the collection of target cells, was 100% and 2%, respectively. It was suggested that the proposed device may be able to separate cells of similar size with high accuracy.