RyhB, an iron-responsive small RNA molecule, regulates Shigella dysenteriae virulence
RyhB, an iron-responsive small RNA molecule, regulates Shigella dysenteriae virulence
复制标题
DOI：
10.1128/iai.00112-07
复制
发表时间：
2007-07-01
影响因子：
3.1
通讯作者：
Payne, Shelley M.
中科院分区：
文献类型：
--
作者：
Murphy, Erin R.;Payne, Shelley M.
小RNA（sRNA）分子对细菌基因表达的调控是一个越来越被认识到的现象，但尚未完全理解。我们发现，sRNA RyhB抑制几个毒力相关的表型志贺氏菌，细菌性痢疾的病原体在人类。S.拟南芥RyhB包括编码III型分泌装置、其分泌的效应子和特异性分子伴侣的那些。志贺氏菌毒力的抑制通过转录激活因子VirB的RyhB依赖性抑制发生，导致VirB调节子内基因表达减少。virB的有效抑制是由RyhB的单链区域介导的，该区域不同于志贺氏菌sodB抑制所需的区域。RyhB对virB的调节暗示铁是一种环境因素，有助于志贺菌毒力决定因子的复杂调节。
Regulation of bacterial gene expression by small RNA (sRNA) molecules is an increasingly recognized phenomenon but one that is not yet fully understood. We show that the sRNA RyhB suppresses several virulence-associated phenotypes of Shigella dysenteriae, a causative agent of bacillary dysentery in humans. The virulence genes repressed by S. dysenteriae RyhB include those encoding the type III secretion apparatus, its secreted effectors, and specific chaperones. Suppression of Shigella virulence occurs via RyhB-dependent repression of the transcriptional activator VirB, leading to reduced expression of genes within the VirB regulon. Efficient repression of virB is mediated by a single-stranded region of RyhB that is distinct from the region required for repression of Shigella sodB. Regulation of virB by RyhB implicates iron as an environmental factor contributing to the complex regulation of Shigella virulence determinants.