Regulator RcsB Controls Prodigiosin Synthesis and Various Cellular Processes in Serratia marcescens JNB5-1
Regulator RcsB Controls Prodigiosin Synthesis and Various Cellular Processes in Serratia marcescens JNB5-1
复制标题
调节器 RcsB 控制粘质沙雷氏菌 JNB5-1 中灵菌红素的合成和各种细胞过程
DOI：
10.1128/aem.02052-20
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发表时间：
2021-01-01
影响因子：
4.4
通讯作者：
Rao, Zhiming
中科院分区：
文献类型：
--
作者：
Pan, Xuewei;Tang, Mi;Rao, Zhiming
RcsB是Rcs磷酸化介导系统中的一个双组分反应调节因子，在肠杆菌科细菌中具有广泛的调节功能。然而，关于RcsB蛋白在细菌中，特别是在S. marcescens，仍然有限。在这项工作中，我们通过实验阐明了RcsB蛋白参与了S. marcescens，包括灵菌红素的合成，细胞运动性，荚膜多糖的产生，生物膜的形成和耐酸性。此外，RcsB蛋白在这些细胞过程中的调节机制进行了研究。总之，这项工作表明RcsB可能是灵菌红素合成的调节剂，并提供了深入了解RcsB蛋白在S. marcescens。灵菌红素（PG）是一种由粘质沙雷氏菌分泌的红色线性三吡咯色素，因其免疫抑制、抗菌和抗癌特性而受到关注。虽然有几个基因已被证明是重要的灵菌红素的合成，这一细胞过程背后的调控机制的信息仍然有限。在这项工作中，我们确定了转录调控因子RcsB编码基因BVG 90_13250（rcsB）负控制灵菌红素的生物合成。marcescens。破坏rcsB赋予灵菌红素的生产显着增加。这种表型对应于灵菌红素相关的猪操纵子由RcsB转录的负控制，可能是通过与灵菌红素合成正调节因子FlhDC的启动子区域结合。此外，利用转录组学和进一步的实验，我们发现RcsB还控制了其他一些重要的细胞过程，包括游泳和群集运动，荚膜多糖的产生，生物膜的形成，和耐酸性（AR），在S。marcescens。总的来说，这项工作提出，RcsB是一个灵菌红素合成阻遏S。marcescens的研究，并提供了深入了解RcsB的细胞运动，荚膜多糖的生产，并在S. marcescens。RcsB是Rcs磷酸化系统中的一个双组分反应调节因子，在肠杆菌科中发挥着多种调节功能。然而，关于RcsB蛋白在细菌中，特别是在S. marcescens，仍然有限。在这项工作中，我们通过实验阐明了RcsB蛋白参与了S. marcescens，包括灵菌红素的合成，细胞运动性，荚膜多糖的产生，生物膜的形成和耐酸性。此外，RcsB蛋白在这些细胞过程中的调节机制进行了研究。总之，这项工作表明RcsB可能是灵菌红素合成的调节剂，并提供了深入了解RcsB蛋白在S. marcescens。
RcsB is a two-component response regulator in the Rcs phosphorelay system, and it plays versatile regulatory functions in Enterobacteriaceae. However, information on the function of the RcsB protein in bacteria, especially in S. marcescens, remains limited. In this work, we illustrated experimentally that the RcsB protein was involved in diverse cellular processes in S. marcescens, including prodigiosin synthesis, cell motility, capsular polysaccharide production, biofilm formation, and acid resistance. Additionally, the regulatory mechanism of the RcsB protein in these cellular processes was investigated. In conclusion, this work indicated that RcsB could be a regulator for prodigiosin synthesis and provides insight into the function of the RcsB protein in S. marcescens. ABSTRACT Prodigiosin (PG), a red linear tripyrrole pigment normally secreted by Serratia marcescens, has received attention for its reported immunosuppressive, antimicrobial, and anticancer properties. Although several genes have been shown to be important for prodigiosin synthesis, information on the regulatory mechanisms behind this cellular process remains limited. In this work, we identified that the transcriptional regulator RcsB encoding gene BVG90_13250 (rcsB) negatively controlled prodigiosin biosynthesis in S. marcescens. Disruption of rcsB conferred a remarkably increased production of prodigiosin. This phenotype corresponded to negative control of transcription of the prodigiosin-associated pig operon by RcsB, probably by binding to the promoter region of the prodigiosin synthesis positive regulator FlhDC. Moreover, using transcriptomics and further experiments, we revealed that RcsB also controlled some other important cellular processes, including swimming and swarming motilities, capsular polysaccharide production, biofilm formation, and acid resistance (AR), in S. marcescens. Collectively, this work proposes that RcsB is a prodigiosin synthesis repressor in S. marcescens and provides insight into the regulatory mechanism of RcsB in cell motility, capsular polysaccharide production, and acid resistance in S. marcescens. IMPORTANCE RcsB is a two-component response regulator in the Rcs phosphorelay system, and it plays versatile regulatory functions in Enterobacteriaceae. However, information on the function of the RcsB protein in bacteria, especially in S. marcescens, remains limited. In this work, we illustrated experimentally that the RcsB protein was involved in diverse cellular processes in S. marcescens, including prodigiosin synthesis, cell motility, capsular polysaccharide production, biofilm formation, and acid resistance. Additionally, the regulatory mechanism of the RcsB protein in these cellular processes was investigated. In conclusion, this work indicated that RcsB could be a regulator for prodigiosin synthesis and provides insight into the function of the RcsB protein in S. marcescens.