Delayed Fourier transform proton magnetic resonance spectroscopy
Delayed Fourier transform proton magnetic resonance spectroscopy
复制标题
延迟傅里叶变换质子磁共振波谱
DOI：
10.1021/ja00762a071
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发表时间：
1972
影响因子：
15
通讯作者：
M. Hsu
中科院分区：
文献类型：
--
作者：
C. Seiter;G. Feigenson;S. Chan;M. Hsu
这种过滤效应的优点可以从我们最近对未超声处理的卵磷脂双层的核磁共振工作中看出。鸡蛋卵磷脂双层样品中质子的典型 CWpmr 谱如图 1a 所示。该光谱揭示了几个狭窄的成分，但信号的大部分非常宽，并且掩盖了更尖锐的共振，使得很难确定它们的光谱位置和强度。图 1b 显示了二棕榈酰卵磷脂在 100 MHz 和 60 MHz（设置为 500 µ 秒）下的延迟傅里叶变换 (DFT) 光谱。在该光谱中，可以看到通常 CW 光谱中的宽分量基本上已完全“过滤”，并且可以轻松监控较窄的分量。 DFT 技术使我们能够监测二棕榈酰卵磷脂中的结晶双层转变。高于 42 ℃，二棕榈酰卵磷脂在过量的 D20 中形成双层分散体，但低于该温度则呈现结晶相。 2 对于结晶相，
The advantage of this filtering effect may be seen in our recent nmr work on unsonicated lecithin bilayers. A typical CWpmr spectrum for the protons in a sample of egg lecithin bilayers is shown in Figure la. This spectrum reveals several narrow components, but the bulk of the signal is very broad, and obscures the sharper resonances, making it difficult to determine their spectral position and intensity. A delayed Fourier transform (DFT) spectrum of dipalmitoyllecithin at 100 MHz and at 60 with set at 500 µ sec is shown in Figure lb. In this spectrum the broad component in the usual CW spectrum can be seen to be essentially completely “filtered,” and the narrower com-ponents can be readily monitored. The DFT technique has enabled us to monitor the crystalline bilayer transition in dipalmitoyllecithin. Above 42, dipalmitoyllecithin forms a bilayer dispersion in excess D20, but below this temperature it ex-hibits a crystalline phase. 2 For the crystalline phase,