Identification of transferred chicken germ cells in quail gonad and semen by amplification of chicken-specific PCR products
Identification of transferred chicken germ cells in quail gonad and semen by amplification of chicken-specific PCR products
复制标题
DOI：
10.2141/jpsa.38.308
复制
发表时间：
2001-10-01
影响因子：
1.5
通讯作者：
Ono, Tamao
中科院分区：
文献类型：
--
作者：
Li, Hai-Chang;Zhao, Li;Ono, Tamao
利用鸡Z染色体上的微卫星位点LEI 0171（GenBank登录号X85538）的PCR引物，在鸡（Gallus arcticus）中扩增出363 bp的基因组DNA片段，但在鹌鹑（Coturnix japonica）中没有扩增出。反应混合物中至少10 pg/穆尔鸡DNA的浓度导致PCR产物的可检测扩增。将鸡胚的循环原始生殖细胞（PGC）输注到鹌鹑胚胎中，从6日龄全胚、10日龄和15日龄胚胎性腺、5日龄雏鸡性腺和成年鸡精液中提取DNA，在鸡-PGC嵌合体鹌鹑中观察到鸡基因组特异性PCR产物。
A polymerase chain reaction (PCR) primer set for a chicken microsatellite locus on the Z chromosome, LEI0171 (GenBank Accession No. X85538), amplified a 363 bp fragment of genomic DNA in chickens (Gallus domesticus) but not in quail (Coturnix japonica). A concentration of at least 10 pg/mul of chicken DNA in the reaction mixture resulted in detectable amplification of the PCR product. Circulating primordial germ cells (PGCs) of chick embryos were transfused into quail embryos, and the chicken-genome-specific PCR product was observed in the chick-PGC-transfused chimeric quail when DNA samples were extracted from whole day-6 embryos, day-10 and -15 embryonic gonads, gonads of 5-day chicks, and the semen of adults.