Immunological memory and neutralizing activity to a single dose of COVID-19 vaccine in previously infected individuals.
Immunological memory and neutralizing activity to a single dose of COVID-19 vaccine in previously infected individuals.
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DOI：
10.1016/j.ijid.2021.05.034
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发表时间：
2021-07
期刊：
影响因子：
--
通讯作者：
Reddy DN
中科院分区：
文献类型：
--
作者：
Sasikala M;Shashidhar J;Deepika G;Ravikanth V;Krishna VV;Sadhana Y;Pragathi K;Reddy DN
此前尚未研究COVID-19疫苗在接种后产生免疫记忆的功效。评估先前SARS-CoV-2感染个体在单剂量mRNA疫苗后的免疫记忆。在获得书面知情同意书后，纳入了医疗工作者（n = 280），并将其分为既往感染和既往无暴露组（分别为逆转录-聚合酶链反应阳性和阴性）。在基线和接种后（COVISHIELD单次给药）采血，通过化学发光计数中和抗体，通过流式细胞术计数记忆T细胞和B细胞。接种疫苗后，与先前未接触组相比，先前感染组有更高水平的：抗体反应（1124.73 ± 869.13 vs 94.23 ± 140.06 Au/ml，p = 0.0001）; CD 4记忆T细胞：中央记忆CCR 7 + CD 45 RA-（p = 0.0001）、效应记忆CCR 7-/CD 45 RA-（p = 0.01）;总CD 8 + T细胞（p = 0.004）; CD 8+初始T细胞CCR 7 + CD 45 RA+（p = 0.01）;和记忆B细胞CD 20 + CD 27+（p = 0.0001）。单剂量疫苗接种在从SARS-CoV-2感染中恢复的个体中引起了更高的中和抗体应答和保护性免疫，与先前未接触过的个体相比。
The efficacy of COVID-19 vaccines to generate immunological memory post-vaccination has not previously been studied. To assess immunological memory in previously SARS-CoV-2 infected individuals after a single dose of mRNA vaccine. Healthcare workers (n = 280) were enrolled after obtaining written informed consent and grouped under previously infected and no prior exposure (reverse transcription-polymerase chain reaction positive and negative, respectively). Blood was drawn at baseline and post-vaccination (single dose of COVISHIELD) for enumerating neutralizing antibodies by chemiluminescence and memory T- and B-cells by flow cytometry. Post vaccination, compared with the no prior exposure group, the previously infected group had higher levels of: antibody response (1124.73 ± 869.13 vs 94.23 ± 140.06 AU/ml, p = 0.0001); CD4 memory T-cells: central memory CCR7+CD45RA- (p = 0.0001), effector memory CCR7-/CD45RA- (p = 0.01); total CD8+ T-cells (p = 0.004); CD8+ naïve T-cells CCR7+CD45RA+ (p = 0.01); and memory B-cells CD20+CD27+ (p = 0.0001). Single-dose vaccination elicited higher neutralizing antibody response and protective immunity in individuals who had recovered from SARS-CoV-2 infection compared with those with no prior exposure.
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DOI：
10.15585/mmwr.mm7009e3
发表时间：
2021-03-05
期刊：
MMWR. Morbidity and mortality weekly report
影响因子：
--
作者：
Rinott E;Youngster I;Lewis YE
通讯作者：
Lewis YE
前沿技术：结核病患者不同感染部位的人体组织驻留记忆 T 细胞的表征
影响因子：
4.4
作者：
Yang, Qianting;Zhang, Mingxia;Ma, Li
通讯作者：
Ma, Li
DOI：
10.1126/science.abf4063
发表时间：
2021-02-05
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Dan JM;Mateus J;Kato Y;Hastie KM;Yu ED;Faliti CE;Grifoni A;Ramirez SI;Haupt S;Frazier A;Nakao C;Rayaprolu V;Rawlings SA;Peters B;Krammer F;Simon V;Saphire EO;Smith DM;Weiskopf D;Sette A;Crotty S
通讯作者：
Crotty S
影响因子：
64.5
作者：
Rodda LB;Netland J;Shehata L;Pruner KB;Morawski PA;Thouvenel CD;Takehara KK;Eggenberger J;Hemann EA;Waterman HR;Fahning ML;Chen Y;Hale M;Rathe J;Stokes C;Wrenn S;Fiala B;Carter L;Hamerman JA;King NP;Gale M Jr;Campbell DJ;Rawlings DJ;Pepper M
通讯作者：
Pepper M
影响因子：
9.2
作者：
Shang, Weilong;Yang, Yi;Rao, Xiancai
通讯作者：
Rao, Xiancai