Specificity and nonspecificity in RNA-protein interactions.
Specificity and nonspecificity in RNA-protein interactions.
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DOI：
10.1038/nrm4032
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发表时间：
2015-09
期刊：
影响因子：
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通讯作者：
Harris ME
中科院分区：
文献类型：
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作者：
Jankowsky E;Harris ME
基因表达受rna和蛋白质相互作用的复杂网络调控。与RNA相互作用的蛋白质传统上被认为是特异性的或非特异性的；特异性蛋白质优先与确定的RNA序列或结构基序相互作用，而非特异性蛋白质与缺乏这些特征的RNA位点相互作用。最近的研究表明，“特异性与非特异性”的二元分类不足以描述rna -蛋白质相互作用的全谱。在这里，我们回顾了能够定量测量蛋白质与大量RNA变体结合的新方法，以及旨在描述由此产生的结合谱的概念：亲和力分布、综合结合模型和自由能景观。我们讨论了这些新方法和相关概念如何使工作朝着包容性，特异性和非特异性rna -蛋白质相互作用的定量模型。
Gene expression is regulated by complex networks of interactions between RNAs and proteins. Proteins that interact with RNA have been traditionally viewed as either specific or non-specific; specific proteins interact preferentially with defined RNA sequence or structure motifs, whereas non-specific proteins interact with RNA sites devoid of such characteristics. Recent studies indicate that the binary “specific vs. non-specific” classification is insufficient to describe the full spectrum of RNA–protein interactions. Here, we review new methods that enable quantitative measurements of protein binding to large numbers of RNA variants, and the concepts aimed as describing resulting binding spectra: affinity distributions, comprehensive binding models and free energy landscapes. We discuss how these new methodologies and associated concepts enable work towards inclusive, quantitative models for specific and non-specific RNA–protein interactions.