Synergistic defense system by cooperative natural effectors against metastasis of B16 melanoma cells in H-2-associated control: different behavior of H-2+ and H-2- cells in metastatic processes.
Synergistic defense system by cooperative natural effectors against metastasis of B16 melanoma cells in H-2-associated control: different behavior of H-2+ and H-2- cells in metastatic processes.
复制标题
H-2相关控制中协同自然效应器对抗B16黑色素瘤细胞转移的协同防御系统：H-2和H-2-细胞在转移过程中的不同行为。
DOI：
10.4049/jimmunol.136.12.4729
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发表时间：
1986
影响因子：
4.4
通讯作者：
K. Nomoto
中科院分区：
文献类型：
--
作者：
Y. Kawano;K. Taniguchi;A. Toshitani;K. Nomoto
通过反复荧光激活细胞分选建立B16黑色素瘤H-2+和H-2-细胞。H-2-系在未处理的C57 BL/6小鼠中未形成转移，而H-2+细胞显示出转移发展的证据。这种差异主要归因于H-2-细胞对天然效应机制攻击的敏感性增加，特别是去唾液酸GM 1 + NK细胞。用抗脱唾液酸GM 1血清和全身照射（400 rad）治疗后，肺部形成大量H-2细胞集落，而照射仅轻微增强转移，用抗脱唾液酸GM 1血清治疗则中度增强转移。对于H-2+细胞，用每种方式处理显著增加了转移性集落的数量。因此，脱唾液酸GM 1 + NK细胞和放射敏感性天然效应物的协作似乎是参与防御H-2-细胞转移的协同效应的主要机制，并且在较小程度上是防御H-2+细胞转移的协同效应。照射（1000拉德）右肺，以消除器官相关的防御增加了集落，特别是在H-2+细胞。另一方面，用抗脱唾液酸GM 1血清治疗增加了转移早期H-2-细胞的定植，并且可能已经消除了能够识别H-2抗原表达减少并在血生期消除H-2-细胞的脱唾液酸GM 1 + NK细胞。自然防御机制可能对H-2+细胞的转移产生抑制作用，主要是在外渗后的器官相关阶段。
H-2+ and H-2- cells of B16 melanoma were established by repeated fluorescence-activated cell sorting. The H-2- line formed no metastasis in untreated C57BL/6 mice, whereas the H-2+ cells showed evidence of metastatic development. This difference was ascribed mainly to the increased susceptibility of H-2- cells to attack by natural effector mechanisms, particularly asialo GM1+ NK cells. After treatment with both anti-asialo GM1 serum and whole body irradiation (400 rad), numerous colonies of H-2- cells formed in the lung, whereas the metastasis was only marginally enhanced by irradiation and moderately by treatment with anti-asialo GM1 serum. With the H-2+ cells, treatment with each modality significantly increased the number of metastatic colonies. Therefore collaboration of asialo GM1+ NK cells and radiosensitive natural effectors seems to be the main mechanism involved in the synergistic effects on defense against H-2- cell metastasis, and to a lesser extent against H-2+ cell metastasis. Irradiation (1000 rad) to the right lung to abrogate the organ-associated defense increased the colonies, particularly in the H-2+ cells. On the other hand, treatment with anti-asialo GM1 serum increased colonization in the early phase of metastasis with H-2- cells and may have abolished asialo GM1+ NK cells capable of recognizing the reduced expression of H-2 antigens and eliminating H-2- cells in the blood-born phase. Natural defense mechanisms probably exert suppressive effects on the metastasis of H-2+ cells, mainly in the organ-associated phase after extravasation.