Nutrition and water temperature regulate the expression of heat-shock proteins in golden pompano larvae (Trachinotus ovate, Limmaeus 1758)
Nutrition and water temperature regulate the expression of heat-shock proteins in golden pompano larvae (Trachinotus ovate, Limmaeus 1758)
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营养和水温调节金鲳鱼幼虫（Trachinotus ovate, Limmaeus 1758）热休克蛋白的表达
DOI：
10.1007/s10695-018-0578-x
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发表时间：
2018
影响因子：
2.9
通讯作者：
马振华
中科院分区：
文献类型：
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作者：
Allais L. Zhao C. Fu M. Hu J. Qin J.G. Qiu L.;马振华
了解鱼类幼体发育对提高水产养殖生产效率具有重要意义。确定可能的指标，鱼苗压力可以提高生产和限制死亡率，幼虫阶段受到。热休克蛋白（HSP）和热休克因子（HSF）是众所周知的对多种应激源（例如，环境、形态或病理变化）。在这项研究中，将金鲷幼虫在不同的温度（23 °C、26 °C和29 °C）以及三种不同的饮食（未富集的卤虫幼虫、富集有微绿球藻的卤虫幼虫、用Algamac 3080富集的卤虫无尾幼体（Artemianauplii），检测HSP 60、HSP 70、HSP 1、HSP 2和GRP 94的表达。应激基因在仔鱼组织中广泛表达，HSP 60和HSP 70主要表达于鳃和心脏，HSP 1主要表达于肌肉、脑和心脏，而GRP 94主要表达于头肾和脾脏。结果表明，金鲳仔鱼对后期温度变化的敏感性较高，29 °C可能是金鲳仔鱼发育的最佳条件;微绿球藻富集卤虫无节幼体处理是金鲳仔鱼最适宜的饵料类型，HSP 60、HSP 70、HSF 1、HSF 2和GRP 94的相对表达量适中。
Understanding fish larval development is of a great interest for aquaculture production efficiency. Identifying possible indicators of fish larvae stress could improve the production and limit the mortality rate that larval stage is subjected to. Heat-shock proteins (HSPs) and heat-shock factors (HSFs) are well known as indicators of response to many kinds of stressor (e.g., environmental, morphological, or pathological changes). In this study, golden pompano larvae were raised at different temperatures (23 °C, 26 °C, and 29 °C), as well as three different diets (Artemianauplii unenriched,Artemianauplii enriched withNannochloropsissp., andArtemianauplii enriched with Algamac 3080), and the expression ofHSP60,HSP70,HSF1,HSP2, andGRP94were monitored. While stress genes were widely expressed in the larval tissues,HSP60andHSP70were principally from the gills and heart;HSF1principally from the muscle, brain, and heart; andGRP94principally from the head kidney and spleen. Golden pompano larvae were found to be more sensitive to thermal changes at later larval stage, and 29 °C was showed to likely be the best condition for golden pompano larval development.Nannochloropsissp.–enrichedArtemia naupliitreatment was found to be the most appropriate feed type with moderate relative expressions ofHSP60,HSP70,HSF1,HSF2, andGRP94.