iTRAQ-Based Proteomic Analysis of Sublethally Injured Escherichia coli O157:H7 Cells Induced by High Pressure Carbon Dioxide.
iTRAQ-Based Proteomic Analysis of Sublethally Injured Escherichia coli O157:H7 Cells Induced by High Pressure Carbon Dioxide.
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基于 iTRAQ 的高压二氧化碳诱导亚致死损伤大肠杆菌 O157:H7 细胞的蛋白质组学分析
DOI：
10.3389/fmicb.2017.02544
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发表时间：
2017
影响因子：
5.2
通讯作者：
Liao X
中科院分区：
文献类型：
--
作者：
Bi X;Wang Y;Hu X;Liao X
高压二氧化碳（HPCD）可引起亚致死损伤细胞（SICs），在食品贮藏过程中可能引起食物中毒和腐败变质，限制了其应用。因此，为了更好地控制HPCD诱导的大肠杆菌O157：H7 SICs的形成，本研究采用iTRAQ蛋白质组学方法对大肠杆菌O157：H7 SICs的形成进行了研究。iTRAQ共鉴定了2，446种蛋白质，其中93种和29种分别与活对照细胞（CKL）和死对照细胞（CKD）相比在SICs中显著差异表达。在93个差异表达蛋白（DEP）中，65个蛋白表达下调，28个蛋白表达上调。综合蛋白质组覆盖率分析表明，SICs在HPCD下通过减少碳水化合物分解、脂质转运和代谢、氨基酸转运和代谢、转录和翻译、DNA复制和修复等途径存活。此外，SICs还表现出应激反应、DNA损伤反应以及对HPCD的糖转运、肽聚糖合成和二硫键形成的增加。在29个DEP中，与CKD相比，SICs中有12个蛋白表达下调，17个蛋白表达上调。综合蛋白质组覆盖率分析表明，SICs在HPCD下通过积累碳水化合物和氨基酸等细胞保护剂，降低转录和翻译活性而存活。结果表明，形成代谢活性低、存活能力强的SICs是大肠杆菌的一种生存策略。coli O157：H7对HPCD的抗性。
High pressure carbon dioxide (HPCD) could cause sublethally injured cells (SICs), which may cause food poisoning and spoilage during food storage and limit its application. Therefore, the formation of SICs of Escherichia coli O157:H7 was investigated by isobaric tag for relative and absolute quantification (iTRAQ) proteomic methods in this study for better controlling the SICs induced by HPCD. A total of 2,446 proteins was identified by iTRAQ, of which 93 and 29 were significantly differentially expressed in the SICs compared with live control cells (CKL) and dead control cells (CKD), respectively. Among the 93 differentially expressed proteins (DEP) in the SICs compared with CKL, 65 proteins showed down-regulation and 28 showed up-regulation. According to the comprehensive proteome coverage analysis, the SICs survived under HPCD by reducing carbohydrate decomposing, lipid transport and metabolism, amino acid transport and metabolism, transcription and translation, DNA replication and repair. Besides, the SICs showed stress response, DNA damage response and an increased carbohydrate transport, peptidoglycan synthesis and disulfide bond formation to HPCD. Among the 29 DEP in the SICs compared with CKD, 12 proteins showed down-regulation and 17 showed up-regulation. According to the comprehensive proteome coverage analysis, the SICs survived under HPCD by accumulation of cell protective agents like carbohydrates and amino acids, and decreasing transcription and translation activities. Results showed that the formation of the SICs with low metabolic activity and high survival ability was a survival strategy for E. coli O157:H7 against HPCD.