Comparison of Heterosubtypic Protection in Ferrets and Pigs Induced by a Single-Cycle Influenza Vaccine
Comparison of Heterosubtypic Protection in Ferrets and Pigs Induced by a Single-Cycle Influenza Vaccine
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DOI：
10.4049/jimmunol.1800142
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发表时间：
2018-06-15
影响因子：
4.4
通讯作者：
Tchilian, Elma
中科院分区：
文献类型：
--
作者：
Holzer, Barbara;Morgan, Sophie B.;Tchilian, Elma
流感是一个主要的健康威胁，广泛保护流感疫苗将是一个重大进展。Signal Minus FLU（S-FLU）是一种候选的广泛保护性流感疫苗，仅限于单循环复制，在鼻内或气雾剂给药后诱导强烈的交叉反应性T细胞应答，但对血凝素的抗体应答最低。我们测试了H3 N2 S-FLU是否可以保护猪和雪貂免受H1N1亚型流感攻击。对猪进行S-FLU气雾剂给药可诱导肺组织驻留记忆T细胞，并减少肺病理学，但不减少病毒载量。相反，在雪貂中，S-FLU减少了病毒复制和气溶胶传播。我们的数据表明，S-FLU在猪和雪貂中具有不同的保护功效，并且在缺乏Ab的情况下，肺T细胞免疫可以降低疾病的严重程度，而不会减少攻击病毒的复制。
Influenza is a major health threat, and a broadly protective influenza vaccine would be a significant advance. Signal Minus FLU (S-FLU) is a candidate broadly protective influenza vaccine that is limited to a single cycle of replication, which induces a strong cross-reactive T cell response but a minimal Ab response to hemagglutinin after intranasal or aerosol administration. We tested whether an H3N2 S-FLU can protect pigs and ferrets from heterosubtypic H1N1 influenza challenge. Aerosol administration of S-FLU to pigs induced lung tissue-resident memory T cells and reduced lung pathology but not the viral load. In contrast, in ferrets, S-FLU reduced viral replication and aerosol transmission. Our data show that S-FLU has different protective efficacy in pigs and ferrets, and that in the absence of Ab, lung T cell immunity can reduce disease severity without reducing challenge viral replication.