Production of singlet oxygen by Ru(dpp(SO3)2)3 incorporated in polyacrylamide PEBBLES
Production of singlet oxygen by Ru(dpp(SO3)2)3 incorporated in polyacrylamide PEBBLES
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DOI：
10.1016/s0925-4005(03)00057-1
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发表时间：
2003-04-20
影响因子：
8.4
通讯作者：
Kopelman, R
中科院分区：
文献类型：
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作者：
Moreno, MJ;Monson, E;Kopelman, R
制备了二磺化4，7-二苯基-1，10-菲咯啉Ru(DPP(SO3)(2))(3)的聚丙烯酰胺(PAA)和胺功能化PAA(AFPAA)纳米粒子。所制备的纳米粒子的流体力学半径为20-25 nm。用菲-9，10-二丙酸(ADPA)测定了Ru(DPP(SO3)(2))(3)产生的单线态氧(O-1(2))的量。建立了Ru(DPP(SO3)(2))(3)在465 nm的稳态辐射下ADPA消失的动力学模型，该模型还考虑了非O-1(2)的消耗。ADPA的这种直接消耗量是通过在NaN3存在的情况下照射来评估的，约占总量的30%。所建立的模型很好地描述了自由Ru(DPP(SO3)(2))(3)和这种染料加入到纳米粒子中的实验结果，发现聚丙烯酰胺基质不会猝灭产生的O-1(2)，使其能够到达纳米粒子的外部溶液并与ADPA反应。当基质含有胺基AFPAA时，与ADPA反应的O-1(2)的量略有减少，为60%，但大多数产生的O-1(2)仍然可以离开颗粒并与外部分子反应。因此，产生的颗粒可用作癌症光动力疗法(PTD)中O-1(2)的来源。这些纳米粒子不会显著猝灭O-1(2)，这一事实使基于PAA纳米粒子并封闭适当传感器分子的O-1(2)传感器的未来发展成为可能。(C)2003 Elsevier Science B.V.保留所有权利。
Polyacrylamide (PAA) and amine-functionalized PAA (AFPAA) nanoparticles with disulfonated 4,7-diphenyl-1,10-phenantroline ruthenium (Ru(dpp(SO3)(2))(3)) have been prepared. The nanoparticles produced have a hydrodynamic radius of 20-25 nm.The amount of singlet oxygen (O-1(2)) produced by Ru(dpp(SO3)(2))(3) as been measured using anthracene-9,10-dipropionic acid (ADPA). A kinetic model for the disappearance of ADPA, by steady state irradiation of Ru(dpp(SO3)(2))(3) at 465 nm, has been developed taking also into account a consumption not mediated by O-1(2). This direct consumption of ADPA is evaluated by irradiating in the presence of NaN3 and is about 30% of the total. All the experimental results are very well described by the model developed, both for free Ru(dpp(SO3)(2))(3) and with this dye incorporated in the nanoparticles.It is found that the polyacrylamide matrix does not quench the O-1(2) produced, allowing it to reach the external solution of the nanoparticles and react with ADPA. When the matrix possesses amine groups, AFPAA, the amount of O-1(2) that reacts with ADPA is slightly reduced, 60%, but most of the O-1(2) produced can still leave the particles and react with external molecules. The particles produced may therefore be used as sources of O-1(2) in photodynamic therapy (PTD) of cancers. The fact that those nanoparticles do not quench significantly the O-1(2) makes possible the future development of O-1(2)) sensors based on PAA nanoparticles with the appropriate sensor molecule enclosed. (C) 2003 Elsevier Science B.V. All rights reserved.