Urokinase‐type plasminogen activator and its inhibitor secreted by cultured human monocyte‐macrophages
Urokinase‐type plasminogen activator and its inhibitor secreted by cultured human monocyte‐macrophages
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培养的人单核巨噬细胞分泌的尿激酶型纤溶酶原激活剂及其抑制剂
DOI：
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发表时间：
1985
影响因子：
5.6
通讯作者：
A. Vaheri
中科院分区：
文献类型：
--
作者：
O. Saksela;T. Hovi;A. Vaheri
培养的人血单核细胞在 1 周内分化为巨噬细胞样细胞。与这种形态转变同时发生，细胞开始释放越来越多的尿激酶（u-PA）型丝氨酸蛋白酶纤溶酶原激活剂（PA；Mr 56,000）和蛋白酶抑制剂α-2-巨球蛋白（α2M）。与细胞相关的 PA 活性（在新鲜单核细胞中也很容易检测到）不同，分泌到无血清培养基中的活性只有在用十二烷基硫酸钠处理样品后才能测量。对培养基进行热或酸处理不足以揭示 PA 活性，这表明除了 α2M 之外，培养基中还存在另一种 PA 抑制物质。该抑制剂 (Mr 65,000) 由巨噬细胞合成，特异性抑制 u-PA 活性，但不抑制组织型 PA (t-PA) 或纤溶酶活性。地塞米松减少分化巨噬细胞的 PA 分泌，而不影响 α2M 或 PA 抑制剂的产生。当添加到单核细胞期细胞中时，地塞米松还抑制细胞的形态分化。
Human blood monocytes in culture differentiate to macrophagelike cells within 1 week. Coinciding with this morphological transition the cells started releasing increasing amounts of the serine proteinase plasminogen activator (PA; Mr 56,000) of the urokinase (u‐PA) type and the proteinase inhibitor alpha‐2‐macroglobulin (α2M). Unlike the cell‐associated PA activity, which was also readily detected in fresh monocytes, the activity secreted into the serum‐free culture medium could be measured only after treatment of the samples with sodium dodecyl sulphate. Heat or acid treatment of the medium was not sufficient to reveal the PA activity, suggesting that, apart from α2M, another PA‐inhibiting substance was present in the culture medium. The inhibitor (Mr 65,000) was found to be synthesized by macrophages and specifically inhibited u‐PA activity but not tissue‐type PA (t‐PA) or plasmin activity. Dexamethasone decreased the secretion of PA by differentiated macrophages without affecting the production of α2M or the PA inhibitor. Dexamethasone also inhibited the morphological differentiation of the cells when added to the monocyte‐phase cells.
DOI：
10.1073/pnas.80.10.2956
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
LOSKUTOFF, DJ;VANMOURIK, JA;LAWRENCE, D
通讯作者：
LAWRENCE, D
在角质形成细胞培养物中产生三种纤溶酶原激活剂和一种抑制剂。
DOI：
10.1016/0304-4165(83)90339-2
发表时间：
1983
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Birkedal-Hansen,H;Taylor,RE
通讯作者：
Taylor,RE