DEGRADATION OF ARABINANS BY ARABINANASES FROM ASPERGILLUS-ACULEATUS AND ASPERGILLUS-NIGER
DEGRADATION OF ARABINANS BY ARABINANASES FROM ASPERGILLUS-ACULEATUS AND ASPERGILLUS-NIGER
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DOI：
10.1016/0144-8617(93)90146-u
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发表时间：
1993-01-01
影响因子：
11.2
通讯作者：
VORAGEN, AGJ
中科院分区：
文献类型：
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作者：
BELDMAN, G;SEARLEVANLEEUWEN, MJF;VORAGEN, AGJ
从一种来源于刺曲霉的酶制剂中纯化出一种阿拉伯糖内酶。经sds -凝胶电泳估计分子量为45 kDa。该酶与具有相同分子量的黑曲霉内阿拉伯糖酶抗体产生反应。除了相似之处，黑曲霉和针叶曲霉的阿拉伯糖内酶也有显著差异。该酶在较高的pH值下活性最佳，与线性阿拉伯糖孵育后产生不同谱的低聚物。这反映在聚合度（DP）为13的低聚物浓度相对较低和DP为6-7的高浓度低聚物。与此相反，黑曲霉阿拉伯糖内酶产生的低聚物浓度逐渐增加，从DP 20增加到DP 3。研究了两种阿拉伯糖内酶，以及来自黑曲霉的阿拉伯糖糠糖苷酶B，对分枝阿拉伯糖的降解进行了研究。阿拉伯糖醛酸苷酶B对阿拉伯糖醛酸侧链的去除基本上与糖苷链的类型无关，但对内阿拉伯糖醛酸酶的消化率有巨大影响。
An endo-arabinanase was purified from an enzyme preparation, derived from Aspergillus aculeatus. After SDS-gel electrophoresis a molecular weight of 45 kDa was estimated. The enzyme was reactive with antibodies raised against endo-arabinanase from Aspergillus niger, having the same molecular weight. Besides similarities, remarkable differences were observed for endo-arabinanases from A. niger and A. aculeatus. The enzyme from A. aculeatus was optimally active at a higher pH and produced a different spectrum of oligomers after incubation with linear arabinan. This was reflected in a relatively low concentration of oligomers with a degree of polymerization (DP) of 13 and a high concentration of oligomers with a DP of 6-7. Contrary to this, the concentration of oligomers produced by the A. niger endo-arabinanase gradually increased, going from DP 20 to DP 3.Both endo-arabinanases, as well as arabinofuranosidase B from A. niger, were studied with respect to the degradation of branched arabinans. Removal of arabinofuranosyl side chains by arabinofuranosidase B was essentially independent of the type of glycosidic linkage but had a tremendous effect on the digestibility by endo-arabinanase.