Peripheral nerve injury and recovery after temporary ischemia
Peripheral nerve injury and recovery after temporary ischemia
复制标题
周围神经损伤及暂时缺血后的恢复
DOI：
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发表时间：
1977
影响因子：
12.7
通讯作者：
H. Teräväinen
中科院分区：
文献类型：
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作者：
J. Mäkitie;H. Teräväinen
摘要在缺血 1、2、3、4 或 6 小时后 1 至 90 天之间检查大鼠坐骨神经的神经 (NCV) 和运动 (MNCV) 传导速度。将结果与神经的光学和电子显微镜进行比较。缺血 1-2 小时后 1 天观察到 MNCV 略有减少，而缺血 3-6 小时则导致完全传导阻滞。 NCV 的减少首先发生在缺血 2 小时后，并且在缺血 4 小时和 6 小时后观察到完全阻断。仅在缺血时间较长（3-6 小时）的动物中，4 至 18 天之间才观察到 NCV 和 MNCV 降低。 NCV 和 MNCV 在第 90 天时几乎恢复正常。缺血4小时和6小时导致一些肌纤维去神经支配，在第4天和第18天表现为自发性纤维性颤动。电子显微镜和组织学研究表明，缺血时间较长后，有髓纤维的变性越来越多；缺血2小时引起约5%、3小时约35%、4小时约45%、6小时约75%的纤维变性。不同尺寸的有髓神经纤维同样受到损伤。在测试的纤维制剂中，可以看到正常的髓鞘和经历沃勒样变性的髓鞘。发生了再生，但即使在第 90 天，髓磷脂/轴突比率也有低于对照值的趋势。
SummaryNerve (NCV) and motor (MNCV) conduction velocities of the rat sciatic nerve were examined between 1 and 90 days after ischemia for 1, 2, 3, 4 or 6 h. The results were compared to light and electron microscopy of the nerve.Slight diminution in the MNCV was observed 1 day after 1–2 h ischemia, whereas 3–6 h ischemia resulted in a complete conduction block. Diminution in the NCV occurred first after ischemia for 2 h and a complete block was seen after 4 and 6 h ischemia. Reduced NCV and MNCV were seen between 4 and 18 days only in the animals subjected to ischemia of longer duration of 3–6 h. Both the NCV and MNCV were nearly normalized at the 90th day. Ischemia of 4 and 6 h resulted in denervation of some of the muscle fibers, seen as spontaneous fibrillation at the 4th and 18th day.Electron microscopy and histometric studies showed degeneration of myelinated fibers increasingly after longer durations of ischemia; ischemia for 2 h caused a degeneration of about 5%, 3 h of about 35%, 4 h about 45%, and 6 h about 75% of the fibers. Myelinated fibers of different sizes were equally damaged. In the teased fiber preparations normal and myelin sheaths undergoing Wallerian-like degeneration was seen. Regeneration occurred, but even at the 90th day there was a tendency of the myelin/axon ratio towards values less than control values.