SELECTION OF LEUKEMIC CELL POPULATIONS BY FREEZING AND THAWING
SELECTION OF LEUKEMIC CELL POPULATIONS BY FREEZING AND THAWING
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DOI：
10.1038/245322a0
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发表时间：
1973-01-01
期刊：
影响因子：
64.8
通讯作者：
MORRIS, GJ
中科院分区：
文献类型：
--
作者：
FARRANT, J;KNIGHT, SC;MORRIS, GJ
白血病患者外周血中正常和异常细胞群的共存可能解释了对有丝分裂原的反应性的变化（由 Ling1 讨论）。一个例子是，与正常细胞相比，慢性淋巴细胞白血病 (CLL) 患者的细胞对植物血凝素 (PHA) 的反应较低或延迟。这些变化可能是由潜在的正常群体对有丝分裂原的反应和异常细胞的不同反应（抑制、无作用或刺激）共同造成的。白血病细胞悬浮液的异质性阻碍了异常细胞群及其相互作用的分析以及血清因子的研究。我们现在已经研究了使用受控冷却条件冷冻和解冻后白血病血液中白血病细胞和正常细胞的存活差异。之前，我们确定了使用二甲亚砜 (DMSO) 作为保护性添加剂时最佳恢复正常人外周淋巴细胞的冷却速率2,3。冷冻前由丝裂原或特定抗原激活的淋巴细胞需要不同的冷却速率才能实现最佳恢复。我们在这里表明，冷却速度比恢复正常淋巴细胞所需的速度更快和更慢，允许白血病血液中异常细胞的不同存活率，并且该技术可以澄清血清因子的研究。
THE co-existence of normal and abnormal cell populations in the peripheral blood of leukaemic patients may explain the variations in the reactivity to mitogens (discussed by Ling1). One example is the low or delayed response of cells from chronic lymphocytic leukaemic (CLL) patients to phytohaemagglutinin (PHA) in comparison with normal cells. The variations presumably result from a combination of the response of the underlying normal population to mitogens and the different responses (inhibition, no effect or stimulation) of the abnormal cells. Analysis of abnormal cell populations and their interactions, together with the study of serum factors, is hindered by the heterogeneity of leukaemic cell suspensions. We have now investigated the differential survival of leukaemic and normal cells from leukaemic blood following freezing and thawing using controlled cooling conditions. Previously we established the cooling rate at which normal human peripheral lymphocytes are recovered optimally using dimethylsulphoxide (DMSO) as a protective additive2,3. Lymphocytes activated by mitogen or specific antigen before freezing required different cooling rates for optimal recovery. We show here that cooling rates both faster and slower than those required for the recovery of normal lymphocytes permit the differential survival of abnormal cells from leukaemic blood and that this technique may clarify the study of serum factors.