Follicle-stimulating hormone signaling and Foxl2 are involved in transcriptional regulation of aromatase gene during gonadal sex differentiation in Japanese flounder, Paralichthys olivaceus.
Follicle-stimulating hormone signaling and Foxl2 are involved in transcriptional regulation of aromatase gene during gonadal sex differentiation in Japanese flounder, Paralichthys olivaceus.
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DOI：
10.1016/j.bbrc.2007.05.208
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发表时间：
2007-08
影响因子：
3.1
通讯作者：
T. Yamaguchi;Sakiko Yamaguchi;T. Hirai;T. Kitano
中科院分区：
文献类型：
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作者：
T. Yamaguchi;Sakiko Yamaguchi;T. Hirai;T. Kitano
牙鲆(Paralichthys Olivaceus)是一种硬骨鱼，具有XX(雌性)/XY(雄性)性别决定系统，表现出依赖温度的性别决定。我们先前已经证明，高水温或芳香酶抑制剂处理会导致遗传雌性向表型雄性的性逆转，并抑制卵巢型P450芳香酶(CYP19A1)的mRNA表达，这是一种负责雄激素转化为雌激素的类固醇合成酶。在本研究中，我们证明了高水温处理特异性地抑制了性分化早期性腺中叉头转录因子基因FOXL2和卵泡刺激素受体(FSHR)的mRNA表达。瞬时转染实验表明，FOXL2和cAMP类似物在体外能够激活细胞色素P19A1基因的转录。这些结果有力地表明，FSH信号和FOXL2参与了温度依赖性性别决定的牙鲆性腺分化过程中CYP19A1基因的转录调控。
Japanese flounder (Paralichthys olivaceus), a teleost fish that has XX (female)/XY (male) sex determination system, exhibits temperature-dependent sex determination. We have previously shown that high water temperature or an aromatase inhibitor treatment causes the sex-reversal from genetic females to phenotypic males and suppression of mRNA expression of ovary-type P450 aromatase (cyp19a1), a steroidogenic enzyme responsible for the conversion of androgens to estrogens, in Japanese flounder. In the present study, we demonstrate that high water temperature treatment suppresses specifically mRNA expression of the forkhead transcription factor gene foxl2, and follicle-stimulating hormone receptor (fshr) in gonads during early sex differentiation. Moreover, transient transfection assay shows that the flounder Foxl2 and cAMP analog can activate the cyp19a1 gene transcription in vitro. These results strongly suggest that FSH signaling and Foxl2 are involved in the transcriptional regulation of cyp19a1 gene during gonadal sex differentiation in Japanese flounder with temperature-dependent sex determination.