Correlation of genetic markers of rejection with biopsy findings following human pancreas transplant
Correlation of genetic markers of rejection with biopsy findings following human pancreas transplant
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DOI：
10.1111/j.1399-0012.2005.00450.x
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发表时间：
2006-01-01
影响因子：
2.1
通讯作者：
Gaber, O
中科院分区：
文献类型：
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作者：
Cashion, A;Sabek, O;Gaber, O
胰腺移植后的急性排斥反应仍然是一个重要的问题，并有助于免疫移植物的损失。胰腺排斥反应的临床指标尚未被普遍接受。最近的研究已经确定了几个细胞毒性基因作为肾和胰岛细胞移植受体急性排斥反应的可能标记。然而，这些排斥标志物尚未在胰腺移植受者中进行评估。本研究评估了颗粒酶B、穿孔素和人白细胞抗原(HLA)-DRA在活检证实的胰腺排斥患者和非活检证实的胰腺排斥患者外周血中的差异表达（每组n = 7）。采用实时逆转录酶聚合酶链反应法分析基因表达水平。这些基因在有和没有1型糖尿病的对照组（n = 17）中的表达也被分析。活检证实胰腺排斥的患者比没有排斥的患者有更高水平的颗粒酶B、穿孔素和HLA-DRA，尽管差异无统计学意义。此外，活检证实胰腺排斥的患者颗粒酶B水平明显高于对照组1型糖尿病患者(p
Acute rejection after pancreas transplantation remains a significant problem and contributes to immunologic graft loss. No clinical markers of pancreas rejection have been universally accepted. Recent studies have identified several cytotoxic genes as possible markers of acute rejection in renal and islet cell transplant recipients. However, these markers of rejection have not been evaluated in pancreas transplant recipients. This study evaluated the differential expression of granzyme B, perforin, and human leukocyte antigen (HLA)-DRA in peripheral blood between patients with and without biopsy-proven pancreas rejection (n = 7 per group). Gene expression levels were analyzed using real time reverse transcriptase polymerase chain reaction assays. Expression of these genes in controls (n = 17) with and without type 1 diabetes was also analyzed. Patients with biopsy-proven pancreas rejection had higher levels of granzyme B, perforin, and HLA-DRA than patients who did not have rejection, although the difference was not statistically significant. Moreover, patients with biopsy-proven pancreas rejection had a significantly higher level of granzyme B than control subjects with type 1 diabetes (p