C/EBPα induces adipogenesis through PPARγ:: a unified pathway
C/EBPα induces adipogenesis through PPARγ:: a unified pathway
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DOI：
10.1101/gad.948702
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发表时间：
2002-01-01
影响因子：
10.5
通讯作者：
Spiegelman, BM
中科院分区：
文献类型：
--
作者：
Rosen, ED;Hsu, CH;Spiegelman, BM
PPARgamma和C/EBPalpha是脂肪形成中的关键转录因子，但这些蛋白质的确切作用一直难以确定，因为它们正调控彼此的表达。问题仍然是这些因素是否独立运作，在独立的，平行的分化途径，或是否存在一个单一的途径。PPARgamma可以促进C/EBPa缺陷细胞中的脂肪形成，但尚未测试相反的情况。我们已经建立了一个永生化的成纤维细胞系，缺乏PPARgamma，我们使用它来显示C/EBPalpha没有能力促进脂肪形成在没有PPARgamma。这些结果表明，C/EBPalpha和PPARgamma参与脂肪细胞发育的单一途径，PPARgamma是脂肪形成的近端效应物。
PPARgamma and C/EBPalpha are critical transcription factors in adipogenesis, but the precise role of these proteins has been difficult to ascertain because they positively regulate each other's expression. Questions remain about whether these factors operate independently in separate, parallel pathways of differentiation, or whether a single pathway exists. PPARgamma can promote adipogenesis in C/EBPalpha-deficient cells, but the converse has not been tested. We have created an immortalized line of fibroblasts lacking PPARgamma, which we use to show that C/EBPalpha has no ability to promote adipogenesis in the absence of PPARgamma. These results indicate that C/EBPalpha and PPARgamma participate in a single pathway of fat cell development with PPARgamma being the proximal effector of adipogenesis.