BAI1-associated protein 2-like 2 is a potential biomarker in lung cancer
BAI1-associated protein 2-like 2 is a potential biomarker in lung cancer
复制标题
BAI1-相关蛋白 2-like 2 是肺癌的潜在生物标志物。
DOI：
10.3892/or.2018.6883
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发表时间：
2019-02-01
期刊：
影响因子：
4.2
通讯作者：
Fu, Xiuhua
中科院分区：
文献类型：
--
作者：
Xu, Lei;Du, Hua;Fu, Xiuhua
肺癌是全球癌症相关死亡的主要原因。引发这种疾病的潜在分子机制在很大程度上仍然未知。I-BAR家族参与调节细胞膜的形成，并且一些成员如BAIAP 2L 1、IRSp 53和MIM已被证明参与肿瘤发生进展。然而，BAI 1相关蛋白2样2（BAIAP 2L 2）在癌症发展中的作用尚不清楚。在本研究中，我们确定BAIAP 2L 2在肺腺癌组织和各种肺癌细胞系中上调。在体外，BAIAP 2L 2沉默导致A549和H1299细胞的活力和集落形成能力降低。相反，BAIAP 2L 2过表达促进95 D细胞的增殖和生长。这些结果表明，BAIAP 2L 2是肺癌细胞增殖和生长所必需的。我们还发现，BAIAP 2L 2基因敲低增加了A549和H1299细胞的凋亡。在分子水平上，BAIAP 2L 2敲低导致许多基因的失调，其中雌激素介导的S期进入途径被显著抑制。总的来说，我们的研究结果揭示了BAIAP 2L 2作为肺癌的新生物标志物和潜在治疗靶点。
Lung cancer is the leading cause of cancer-related death worldwide. The underlying molecular mechanisms that trigger this disease remain largely unknown. The I-BAR family is involved in regulating cell membrane formation and some members, such as BAIAP2L1, IRSp53 and MIM have been shown to participate in tumorigenic progression. However, the role of BAI1-associated protein 2-like 2 (BAIAP2L2) in cancer development is unclear. In the present study, we determined that BAIAP2L2 was upregulated in lung adenocarcinoma tissues and various lung cancer cell lines. In vitro, BAIAP2L2 silencing resulted in decreased viability and colony formation capacity of both A549 and H1299 cells. By contrast, BAIAP2L2 overexpression promoted the proliferation and growth of 95D cells. These results indicated that BAIAP2L2 was essential for lung cancer cell proliferation and growth. We also found that BAIAP2L2 knockdown increased the apoptosis of A549 and H1299 cells. At the molecular level, BAIAP2L2 knockdown led to dysregulation of numerous genes, among which the Estrogen-mediated S-phase Entry pathway was significantly suppressed. Collectively, our findings revealed BAIAP2L2 as a novel biomarker and potential therapeutic target for lung cancer.