MRI-based localization of electrophysiological recording sites within the cerebral cortex at single-voxel accuracy
MRI-based localization of electrophysiological recording sites within the cerebral cortex at single-voxel accuracy
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DOI：
10.1038/nmeth987
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发表时间：
2007-02-01
期刊：
影响因子：
48
通讯作者：
Miyashita, Yasushi
中科院分区：
文献类型：
--
作者：
Matsui, Teppei;Koyano, Kenji W.;Miyashita, Yasushi
在灵长类动物大脑皮层层的微电极记录位置的定位允许分析记录的神经元活动和潜在的解剖连接之间的关系。我们提出了一种磁共振成像方法来精确定位体内皮层记录部位。在这种方法中，磁化率诱导效应使微电极的外观变厚，并增强了微电极尖端的可探测性，而微电极尖端通常只占图像体素体积的百分之几。在模体研究中，优化的磁化率诱导效应允许单体素精度（面内分辨率，50 μ m）的尖端检测。我们将这种方法应用于记录插入猕猴大脑的微电极，并将微电极尖端定位在2 - 3mm厚的皮层内，其面内分辨率为150 μ m。随后的组织学分析证实了体内尖端定位的单体素准确性。该方法为研究大脑认知过程中的信息流开辟了一条途径。
The localization of microelectrode recording sites in the layers of primate cerebral cortex permits the analysis of relationships between recorded neuronal activities and underlying anatomical connections. We present a magnetic resonance imaging method for precise in vivo localization of cortical recording sites. In this method, the susceptibility-induced effect thickens the appearance of the microelectrode and enhances the detectability of the microelectrode tip, which usually occupies less than a few percent of the volume of an image voxel. In a phantom study, the optimized susceptibility-induced effect allowed tip detection with single-voxel accuracy (in-plane resolution, 50 mu m). We applied this method to recording microelectrodes inserted into the brains of macaque monkeys, and localized the microelectrode tip at an in-plane resolution of 150 mu m within the cortex of 2 - 3 mm in thickness. Subsequent histological analyses validated the single-voxel accuracy of the in vivo tip localization. This method opens up a way to investigate information flow during cognitive processes in the brain.