Diurnal change of the cold-inducible RNA-binding protein (Cirp) expression in mouse brain
Diurnal change of the cold-inducible RNA-binding protein (Cirp) expression in mouse brain
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DOI：
10.1006/bbrc.1998.8482
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发表时间：
1998-04-17
影响因子：
3.1
通讯作者：
Fujita, J
中科院分区：
文献类型：
--
作者：
Nishiyama, H;Xue, JH;Fujita, J
rna结合蛋白被认为在神经功能调控中起重要作用。最近，一种编码冷诱导rna结合蛋白Cirp的小鼠cDNA被分离出来，其氨基酸序列与植物昼夜节律蛋白相似。在本研究中，我们检测了Cirp在小鼠神经系统中的日表达。Northern blot分析显示，Cirp mRNA的水平在大脑中受到昼夜调节，而在睾丸和肝脏中没有。该水平在白天上升，在夜间下降。使用抗Cirp抗体免疫组化显示Cirp在神经元核中表达，并且Cirp的水平在视交叉上核和大脑皮层中受到昼夜调节。在持续黑暗条件下的成年小鼠和3日龄小鼠的大脑中均未观察到昼夜调节。这些发现表明，Cirp在植物蛋白的生物节律中起着重要作用，并且Cirp的表达在不同的大脑区域受到不同的调节。(C) 1998学术出版社。
RNA-binding proteins are believed to play important roles in regulation of neural functions. Recently, a mouse cDNA encoding the cold-inducible RNA-binding protein, Cirp, has been isolated, the amino acid sequence of which showed similarity to plant circadian rhythm proteins. In the present study, diurnal expression of Cirp in the mouse nervous system was examined. Northern blot analysis showed that the level of Cirp mRNA was diurnally regulated in the brain but not in the testis and liver. The level increased during the daytime and decreased during the nighttime. Immunohistochemistry using an anti-Cirp antibody showed that Cirp was expressed in the nucleus of neurons and that the level of Cirp was diurnally regulated in the suprachiasmatic nucleus and the cerebral cortex. The diurnal regulation was not observed in the brain of adult mice kept in constant darkness nor that of 3-day-old mice. These findings suggest that Cirp plays a role in biological rhythms as known for plant proteins and that expression of Cirp is regulated differentially in discrete brain regions. (C) 1998 Academic Press.