ALIGNMENT OF U3 REGION SEQUENCES OF MAMMALIAN TYPE-C VIRUSES - IDENTIFICATION OF HIGHLY CONSERVED MOTIFS AND IMPLICATIONS FOR ENHANCER DESIGN
ALIGNMENT OF U3 REGION SEQUENCES OF MAMMALIAN TYPE-C VIRUSES - IDENTIFICATION OF HIGHLY CONSERVED MOTIFS AND IMPLICATIONS FOR ENHANCER DESIGN
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DOI：
10.1128/jvi.64.2.534-542.1990
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发表时间：
1990-02-01
影响因子：
5.4
通讯作者：
HOPKINS, N
中科院分区：
文献类型：
--
作者：
GOLEMIS, EA;SPECK, NA;HOPKINS, N
我们对已发表的35种C型哺乳动物逆转录病毒的U3区序列进行了比对。比对结果表明，U3区域内的某些序列基序非常保守。其中许多主题对应于之前确定的地点。特别是，我们发现大多数病毒的增强子区域包含白血病病毒因子b的结合位点、病毒核心样元件、核因子1的共识基序和糖皮质激素反应元件。大多数包含不止一个增强子序列拷贝的病毒在两个拷贝的重复序列中都包括这些结合位点。我们认为这组结合位点构成了这组病毒增强剂的框架。U3区其他高度保守的基序包括逆转录病毒反向重复序列、负调控元件以及CCAAT和TATA盒。此外，我们在启动子区域发现了两个新的基序，它们非常保守，但以前没有描述过。
We aligned published sequences for the U3 region of 35 type C mammalian retroviruses. The alignment reveals that certain sequence motifs within the U3 region are strikingly conserved. A number of these motifs correspond to previously identified sites. In particular, we found that the enhancer region of most of the viruses examined contains a binding site for leukemia virus factor b, a viral corelike element, the consensus motif for nuclear factor 1, and the glucocorticoid response element. Most viruses containing more than one copy of enhancer sequences include these binding sites in both copies of the repeat. We consider this set of binding sites to constitute a framework for the enhancers of this set of viruses. Other highly conserved motifs in the U3 region include the retrovirus inverted repeat sequence, a negative regulatory element, and the CCAAT and TATA boxes. In addition, we identified two novel motifs in the promoter region that were exceptionally highly conserved but have not been previously described.