Distribution of antifungal proteins in maize kernel tissues using immunochemistry
Distribution of antifungal proteins in maize kernel tissues using immunochemistry
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DOI：
10.4315/0362-028x-62.3.295
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发表时间：
1999-03-01
影响因子：
2
通讯作者：
Russin, JS
中科院分区：
文献类型：
--
作者：
Guo, BZ;Cleveland, TE;Russin, JS
研究了核糖体失活蛋白（RIP）和玉米素（zeamatin）两种抗真菌蛋白在玉米籽粒组织中的分布。从玉米吸胀后的胚乳（包括糊粉层）和胚组织中提取蛋白质。Western blot分析表明，RIP样蛋白是目前在胚乳比在胚组织中的水平较高，而玉米蛋白样蛋白在胚组织比胚乳组织中更集中。Western blot分析表明，胚乳中有3条蛋白带，胚中有2条蛋白带与RIP抗体反应。进行组织印片来定位抗真菌蛋白。将吸胀的籽粒纵向和横向切割，并印迹到硝酸纤维素膜上。利用抗玉米RIP和玉米素的抗体，RIP主要存在于胚乳的糊粉层和盾片的腺体层中，而玉米素主要存在于籽粒胚中。这些结果提供了深入了解这些抗真菌蛋白的潜在功能，特别是因为玉米籽粒中RIP和玉米蛋白的存在独特地保护籽粒免受病原体的侵害。
This study examined the distribution of two antifungal proteins, ribosome-inactivating protein (RIP) and zeamatin, in maize kernel tissues. Proteins were extracted from endosperm (including aleurone layer) and embryo tissues of imbibed maize kernels. Western blot analyses revealed that RIP-like protein was present at higher levels in endosperm than in embryo tissues, whereas zeamatin-like protein was more concentrated in embryo tissues than in endosperm tissues. However, there were three protein bands in the endosperm and two bands in the embryo that reacted to anti-RIP antibody in Western blot analyses. Tissue prints were conducted to localize the antifungal proteins. Imbibed kernels were cut longitudinally and transversely and blotted onto nitrocellulose membranes. Using antibodies against maize RIP and zeamatin, RIP was found primarily in the aleurone layer of the endosperm and glandular layer of scutellum, whereas zeamatin was located mainly in the kernel embryo. These results provide insight into the potential functions of these antifungal proteins, especially since the presence of RIP and zeamatin within maize kernels uniquely protects kernels from pathogens.