Microdosimetry for conventional and supra-electroporation in cells with organelles
Microdosimetry for conventional and supra-electroporation in cells with organelles
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DOI：
10.1016/j.bbrc.2006.01.094
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发表时间：
2006-03-24
影响因子：
3.1
通讯作者：
Weaver, JC
中科院分区：
文献类型：
--
作者：
Gowrishankar, TR;Esser, AT;Weaver, JC
常规电穿孔（EP）通过0.1至lkV/cm脉冲长于100 μ s，和超电穿孔通过10至300 kV/cm脉冲短于11 s引起不同的细胞效应。常规EP递送DNA、蛋白质、小分子药物和荧光指示剂穿过质膜（PM），并在PM处引起中等水平的磷脂酰丝氨酸（PS）易位。我们推测，超EP是中央细胞内的影响，如凋亡诱导和PS易位的水平更高。我们的细胞系统模型具有20，000个相互连接的局部模型，用于PM和细胞器膜的小区域，水介质的小区域，适当的静息电位和渐近EP模型。传统的EP主要影响PM，但暗示内质网参与。在最大的视野下，超EP可以涉及所有细胞膜。常规EP场倾向于围绕细胞，但超EP场穿过细胞，广泛穿透细胞器。(c)2006爱思唯尔公司All rights reserved.
Conventional electroporation (EP) by 0.1 to 1 kV/cm pulses longer than 100 mu s, and supra-electroporation by 10 to 300 kV/cm pulses shorter than I lis cause different cellular effects. Conventional EP delivers DNA, proteins, small drugs, and fluorescent indicators across the plasma membrane (PM) and causes moderate levels of phosphatidylserine (PS) translocation at the PM. We hypothesize that supra-EP is central to intracellular effects such as apoptosis induction and higher levels of PS translocation. Our cell system model has 20,000 interconnected local models for small areas of the PM and organelle membranes, small regions of aqueous media, appropriate resting potentials, and the asymptotic EP model. Conventional EP primarily affects the PM, but with a hint of endoplasmic reticulum involvement. Supra-EP can involve all of a cell's membrane at the largest fields. Conventional EP fields tend to go around cells, but supra-EP fields go through cells, extensively penetrating organelles. (c) 2006 Elsevier Inc. All rights reserved.