Quantitative Proteome Analysis of Multidrug Resistance in Human Ovarian Cancer Cell Line
Quantitative Proteome Analysis of Multidrug Resistance in Human Ovarian Cancer Cell Line
复制标题
人卵巢癌细胞系多药耐药性的定量蛋白质组分析
DOI：
10.1002/jcb.22413
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发表时间：
2010-03-01
影响因子：
4
通讯作者：
Zhang, Da-Zhi
中科院分区：
文献类型：
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作者：
Li, Sang-Lin;Ye, Feng;Zhang, Da-Zhi
为了解卵巢癌多药耐药（MDR）的分子机制，我们采用蛋白质组学方法iTRAQ和LC-MS/MS技术，以顺铂耐药细胞系C 0 C1/DDP及其亲本细胞系C 0 C1为模型。共鉴定出28个差异表达蛋白，然后通过Western印迹分析和/或实时RT-PCR确认部分鉴定的蛋白的差异表达水平。进一步分析PKM 2和HSPD 1两个差异表达蛋白与卵巢癌细胞多药耐药的关系，结果表明PKM 2和HSPD 1在卵巢癌细胞顺铂耐药中起重要作用。差异表达蛋白质按功能可分为钙结合蛋白、分子伴侣、细胞外基质、药物解毒或DNA损伤修复蛋白、代谢酶、转录因子、细胞结构相关蛋白和信号转导相关蛋白等8类。这些结果为进一步研究卵巢癌多药耐药机制提供了重要依据。J.细胞。109：625-633，2010. (C)2010 Wiley-Liss，Inc.
In order to understand the molecular mechanisms of multidrug resistance (MDR) in ovarian cancer, we employed the proteomic approach of isobaric tags for relative and absolute quantification (iTRAQ), followed by LC-MS/MS, using the cisplatin-resistant C0C1/DDP cell line and its parental C0C1 cell line as a model. A total number of 28 proteins differentially expressed were identified, and then the differential expression levels of partially identified proteins were confirmed by Western blot analysis and/or real-time RT-PCR. Furthermore, the association of PKM2 and HSPD1, two differentially expressed proteins, with MDR were analyzed, and the results showed that they could contribute considerably to the cisplatin resistance in ovarian cancer cell. The differential expression proteins could be classified into eight categories based on their functions, that is, calcium-binding proteins, chaperones, extracellular matrix, proteins involved in drug detoxification or repair of DNA damage, metabolic enzymes, transcription factor, proteins related to cellular structure and proteins relative to signal transduction. These data will be valuable for further study of the mechanisms of MDR in the ovarian cancer. J. Cell. Biochem. 109: 625-633, 2010. (C) 2010 Wiley-Liss, Inc.