The transcriptional activity of a temperature-sensitive transcription factor module is associated with pollen shedding time in pine
The transcriptional activity of a temperature-sensitive transcription factor module is associated with pollen shedding time in pine
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温度敏感转录因子模块的转录活性与松树花粉脱落时间相关
DOI：
10.1093/treephys/tpz023
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发表时间：
2019
期刊：
影响因子：
4
通讯作者：
Li Wei
中科院分区：
文献类型：
--
作者：
Niu Shi-Hui;Liu Shuang-Wei;Ma Jing-Jing;Han Fang-Xu;Li Yue;Li Wei
人们早就知道松树的花粉脱落时间与温度有关，但其分子基础在很大程度上仍然未知。为了更好地了解油松花粉温度响应的机制，并确定调控花粉脱落时间的中枢调控因子，我们利用6株早花粉脱落树（EPs）和6株晚花粉脱落树（LPs）在仲冬和早春连续3个时间点进行了比较转录组分析，确定了一组温度敏感基因。我们进行了加权基因共表达网络分析，并构建了一个转录因子（TF）协作网络，合并到一个联合网络中的EP和LP的共同但差异表达的TF。我们在核心TF模块中发现了五个中心基因，其表达被低温快速诱导。该TF模块的转录活性与花粉脱落时间密切相关，并可能在早春产生抗寒性和生长活性之间的良好平衡。我们的研究结果为松树开花时间季节性调控的分子机制提供了新的见解，并将为未来的相关研究提供参考。
It has long been known that the pollen shedding time in pine trees is correlated with temperature, but the molecular basis for this has remained largely unknown. To better understand the mechanisms driving temperature response and to identify the hub regulators of pollen shedding time regulation in Pinus tabuliformis, we identified a set of temperature-sensitive genes by carrying out a comparative transcriptome analysis using six early pollen shedding trees (EPs) and six late pollen shedding trees (LPs) during mid-winter and at three consecutive time points in early spring. We carried out a weighted gene co-expression network analysis and constructed a transcription factor (TF) collaborative network, merging the common but differentially expressed TFs of the EPs and LPs into a joint network. We found five hub genes in the core TF module whose expression was rapidly induced by low temperatures. The transcriptional activity of this TF module was strongly associated with pollen shedding time, and likely to produce the fine balance between cold hardiness and growth activity in early spring. Our results provide new insights into the molecular mechanisms of seasonal flowering time regulation in pines, and will inform related studies in the future.