Self-served and fully automated biochemical detection of finger-prick blood at home using a portable microfluidic analyzer
Self-served and fully automated biochemical detection of finger-prick blood at home using a portable microfluidic analyzer
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DOI：
10.1016/j.snb.2019.127235
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发表时间：
2020-01-15
影响因子：
8.4
通讯作者：
Cheng, Jing
中科院分区：
文献类型：
--
作者：
Zhu, Yunzeng;Meng, Xiangrui;Cheng, Jing
慢性病的负担无疑是全球健康面临的主要挑战。慢性病是一种长期性疾病，其干预需要经常监测病情发展，因此在家进行血液生化自我检查非常必要。然而，目前能够在家中同时进行多种指尖采血生化检测的设备还很少。在本研究中，提出了一种完全集成的微流体分析仪，可以进行多种血液生化分析。指尖采血可直接吸入含有稀释液和冻干试剂的芯片中，血清/血浆分离与计量、稀释液释放与计量、定量血清/血浆与稀释液混合、稀释后的血清/血浆分配至反应室、生化反应和结果测量等过程在自带的微流控芯片上依次自动进行。使用这款一键操作的便携式分析仪，用户无需接受医学培训，即可轻松完成自己的生化检测，使生化层面的慢性病自助健康监测成为可能。目前的报告中，在15分钟内实现了血糖、总胆固醇和甘油三酯等三项血液生化指标的全自动检测。用这种微流控分析仪测量了志愿者的指尖血样，结果与医院使用的商用生化分析仪获得的结果相当。
The burden of chronic diseases is unquestionably a predominant challenge to global health. Chronic diseases are the long-term diseases and their intervention requires frequent monitoring of disease development, which makes the self-checkup of blood biochemistry at home highly desired. However, there is few devices that can be used at home for simultaneous test of multiple finger-prick blood biochemicals. In this study, a fully integrated microfluidic analyzer which can perform multiple blood biochemical analysis is proposed. The finger-prick blood can be directly aspirated into the chip containing diluent and lyophilized reagents, and the following processes, including serum/plasma separation and metering, diluent release and metering, quantified serum/plasma and diluent mixing, the diluted serum/plasma distribution to the reaction chambers, and the biochemical reaction and results measurement, were sequentially and automatically performed on the self-contained microfluidic chip. Using this one key operated portable analyzer, users can easily accomplish their own biochemical detection without medical training, making self-served health monitoring for chronic diseases at biochemical level possible. In the current report, fully automated detection for three blood biochemical indexes, including blood glucose, total cholesterol and triglycerides, were achieved in 15 min. The finger-prick blood samples from volunteers were measured by this microfluidic analyzer, and the results were comparable to those obtained from a commercial biochemical analyzer used in hospital.