An overview of extrusion-based bioprinting with a focus on induced shear stress and its effect on cell viability
An overview of extrusion-based bioprinting with a focus on induced shear stress and its effect on cell viability
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DOI：
10.1016/j.bprint.2020.e00093
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发表时间：
2020-12-01
期刊：
影响因子：
--
通讯作者：
Stefanini, Cesare
中科院分区：
文献类型：
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作者：
Boularaoui, Selwa;Al Hussein, Ghada;Stefanini, Cesare
三维生物打印是组织工程领域的一种新兴制造技术。基于挤出的生物打印是广泛使用的生物制造技术之一;研究人员倾向于使用它，因为它简单，经济实惠和可扩展性。在基于挤出的生物打印过程中，细胞受到不同类型的机械力。在这些机械力中，剪切应力具有特殊的意义和关注，因为它被认为是细胞损伤/死亡的主要原因。本文介绍了挤出生物打印的原理，着重介绍了挤出生物打印过程中产生的剪切应力以及细胞对剪切应力的感知和反应，并讨论了挤出生物打印中常用的生物墨水沿着其特性，这些特性已被证明对过程诱导的剪切应力和细胞活力有影响。此外，还详细讨论了剪切应力、细胞活力与不同材料特性和印刷参数之间的关系。此外，它还提供了成功基于挤出的生物打印3D组织的例子。最后，它揭示了克服剪切应力效应，从而保持细胞活力的方法。
Three dimensional bioprinting is an emerging fabrication technique in the field of tissue engineering. Extrusion-based bioprinting is one of the widely employed biofabrication techniques; researchers tend to use it because of its simplicity, affordability and scalability. During the extrusion-based bioprinting process, cells are subjected to mechanical forces of different kinds. Among these mechanical forces, shear stress is of a special significance and concern as it is considered the main cause of cell damage/death. This review covers the principle of extrusion-based bioprinting, and highlights extrusion-based bioprinting induced shear stress, how cells sense it and respond to it. Moreover, it discusses the bioinks that are commonly used in extrusion-based bioprinting along with their properties that have proven to have an effect on process induced shear stress and cell viability. In addition, it discusses, in detail, the relationship between shear stress, cell viability and different material properties and printing parameters. Furthermore, it provides examples of successfully extrusion-based bioprinted 3D tissues. Finally, it sheds light upon approaches to overcome shear stress effect and therefore preserve cell viability.