Detection of Agrobacterium tumefaciens in grapevine cuttings
Detection of Agrobacterium tumefaciens in grapevine cuttings
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葡萄插条中根癌农杆菌的检测
DOI：
10.1111/j.1365-2338.1987.tb00014.x
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
T. Burr
中科院分区：
文献类型：
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作者：
C. Bazzi;C. Piazza;T. Burr
根癌病是葡萄上最重要的细菌性病害，主要与根癌农杆菌生物群3有关。根癌农杆菌在葡萄上的系统分布需要对苗圃繁殖材料进行检查，以揭示它们在木质部导管中的存在。用来证明系统存活的方法相当冗长，因此，本研究旨在建立一种血清学采样程序，以快速检测从一年无症状的休眠葡萄插条中提取的木质部提取液中的根癌农杆菌。将悬液离心，最后的浓缩物用于免疫荧光染色。对真空洗涤系统的灵敏度阈值和检测方法的有效性进行了实验评价。抗血清特异性在与来自葡萄和其他常见葡萄污染物的根癌农杆菌菌株的比较研究中进行了测试。真空洗涤系统在一项备受期待的认证计划中被讨论为一种索引工具。
Crown gall is the most important bacterial disease of grapevine and is primarily associated with Agrobacterium tumefaciens biovar 3. The systemic distribution of the agrobacteria in grapevine requires the checking of nursery propagation material to reveal their presence in the xylem vessels. The methods adopted to demonstrate systemic survival are quite lengthy and, thus, this study aimed to develop a serological sampling procedure to rapidly detect A. tumefaciens in xylem extracts, obtained by vacuum extraction with suitable washing fluid, from asymptomatic one year-old dormant grape cuttings. The suspension was centrifuged and the final concentrate used for immunofluorescence staining. The sensitivity threshold of the vacuum washing system and the effectiveness of the detection method were experimentally evaluated. The antiserum specificity was tested in a comparative study with a collection of A. tumefaciens strains from grapevine and other common grapevine contaminants. The vacuum washing system is discussed as an indexing tool in a much hoped for certification programme.