EXPRESSION OF THE POTENT INFLAMMATORY CYTOKINES, GRANULOCYTE-MACROPHAGE-COLONY-STIMULATING FACTOR AND INTERLEUKIN-6 AND INTERLEUKIN-8, IN BRONCHIAL EPITHELIAL-CELLS OF PATIENTS WITH ASTHMA
EXPRESSION OF THE POTENT INFLAMMATORY CYTOKINES, GRANULOCYTE-MACROPHAGE-COLONY-STIMULATING FACTOR AND INTERLEUKIN-6 AND INTERLEUKIN-8, IN BRONCHIAL EPITHELIAL-CELLS OF PATIENTS WITH ASTHMA
复制标题
DOI：
10.1016/0091-6749(92)90223-o
复制
发表时间：
1992-05-01
影响因子：
14.2
通讯作者：
MATTOLI, S
中科院分区：
文献类型：
--
作者：
MARINI, M;VITTORI, E;MATTOLI, S
我们以前已经证明，培养的人支气管上皮细胞产生的细胞因子具有强有力的促炎特性，在体外暴露于几种刺激，我们已经假设，这些上皮细胞衍生的因子可能有助于支气管粘膜的一些炎症性疾病的发病机制，特别是哮喘，通过促进粒细胞和T细胞的浸润及其局部激活。在这项研究中，我们提供了直接的证据，粒细胞-巨噬细胞集落刺激因子，白细胞介素-6，白细胞介素-8基因和蛋白质的表达增加，在支气管上皮症状性哮喘患者。皮质类固醇激素（10（-7）mol/L）可在体外阻断哮喘患者支气管上皮细胞产生上述细胞因子的上调，但从病变组织中取出细胞后6天内，上述细胞因子的上调也自发消失。
We have previously demonstrated that cultured human bronchial epithelial cells produce cytokines with potent proinflammatory properties on exposure to several stimuli in vitro, and we have hypothesized that these epithelial cell-derived factors may contribute to the pathogenesis of some inflammatory diseases of the bronchial mucosa, particularly asthma, by promoting the infiltration of granulocytes and T cells and their local activation. We provide, in this study, direct evidence of an increased expression of granulocyte-macrophage-colony-stimulating factor, interleukin-6, and interleukin-8 genes and proteins in bronchial epithelium from patients with symptomatic asthma. The up regulation of the production of these cytokines in bronchial epithelial cells of patients with asthma could be abolished in vitro by corticosteroids (hydrocortisone, 10(-7) mol/L), but the up regulation also spontaneously disappeared during a period of 6 days after the removal of the cells from the diseased tissue.