The effects of intraarticular resiniferatoxin in experimental knee-joint arthritis
The effects of intraarticular resiniferatoxin in experimental knee-joint arthritis
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DOI：
10.1213/01.ane.0000180998.29890.b0
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发表时间：
2005-11-01
影响因子：
5.7
通讯作者：
Kissin, I
中科院分区：
文献类型：
--
作者：
Kissin, EY;Freitas, CF;Kissin, I
在这项研究中，我们试图确定是否在实验性关节炎关节内注射香草素激动剂树脂毒素（RTX）产生镇痛作用。通过关节内角叉菜胶（2%，30 μ L）在大鼠中诱导膝关节炎症。疼痛评分和左/右后腿重量分布比用于评估疼痛行为。通过测量外周和关节内面积（超声扫描）评估膝关节尺寸的变化。RTX（0.0003%或0.003%，30 μ L）关节腔内给药提供了显著的镇痛作用。RTX给药后24小时，疼痛评分从15.1 +/- 4.7降至6.9 +/- 4.4（P < 0.01），降低0.0003%，疼痛消失（P < 0.0001），降低0.003%。RTX给药后，体重分布比的改善持续了几天。膝周长的减少表明关节内RTX抑制角叉菜胶诱导的水肿至少三分之一。超声扫描显示没有RTX引起的关节内面积减少。实验表明，关节内RTX抑制膝关节炎的疼痛行为，并且这种效果是剂量依赖性的。这些结果为外周镇痛提供了一个新的方向。
In this study we sought to determine whether an intraarticular administration of a vanilloid agonist resiniferatoxin (RTX) produces an analgesic effect in experimental arthritis. Knee joint inflammation was induced in rats by intraarticular carrageenan (2%, 30 mu L). Pain score and left/right hind leg weight distribution ratio were used to assess pain behavior. Changes in knee dimensions were evaluated by measuring external circumference and intraarticular area (ultrasound scanning). The intraarticular administration of RTX (0.0003% or 0.003%, 30 mu L) provided a significant analgesic effect. Twenty-four hours after RTX administration, the pain score was reduced from 15.1 +/- 4.7 to 6.9 +/- 4.4 (P < 0.01) with 0.0003% and was abolished (P < 0.0001) with 0.003%. The improvement in weight distribution ratio lasted for several days after the RTX administration. Reduction in knee circumference demonstrated that intraarticular RTX suppressed the carrageenan-induced edema by at least one third. Ultrasound scanning revealed no RTX-induced decrease of the intraarticular area. The experiments demonstrated that intraarticular RTX inhibits pain behavior in knee-joint arthritis and that this effect is dose-dependent. These results suggest a new direction for peripheral analgesia.