Direct astrocytic contacts regulate local maturation of dendritic spines
Direct astrocytic contacts regulate local maturation of dendritic spines
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DOI：
10.1523/jneurosci.4466-06.2007
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发表时间：
2007-01-10
影响因子：
5.3
通讯作者：
Okabe, Shigeo
中科院分区：
文献类型：
--
作者：
Nishida, Hideko;Okabe, Shigeo
星形胶质细胞对突触的发育和功能都有贡献，但星形胶质细胞的直接接触是否调节单个突触的发育仍不清楚。星形胶质细胞和树突状突起活动的双光子延时成像揭示了星形胶质细胞接触与树突状突起的寿命和形态成熟的相关性。星形胶质细胞的运动是必不可少的成熟的棘，因为它的抑制，通过操纵Rac 1依赖的信号在星形胶质细胞导致诱导较长的，丝状伪足样树突状突起。操纵ephrin/Eph依赖的神经元-星形胶质细胞信号转导表明该信号转导通路参与了星形胶质细胞依赖的新产生的树突状突起的稳定。我们的数据支持一个模型，在该模型中，星形胶质细胞在发育过程中的活动作为一个关键的局部调节稳定的个别树突突起和随后的成熟成刺。
Astrocytes contribute on both development and function of synapses, but it remains unclear whether direct astrocytic contacts regulate development of individual synapses. Two-photon time-lapse imaging of astrocytic and dendritic protrusive activity revealed the correlation of astrocytic contacts with both lifetime and morphological maturation of dendritic protrusions. Astrocytic motility was essential in maturation of spines, because its suppression by manipulating Rac1-dependent signaling in astrocytes resulted in induction of longer, filopodia-like dendritic protrusions. Manipulation of ephrin/Eph-dependent neuron-astrocyte signaling suggested involvement of this signaling pathway in astrocyte-dependent stabilization of newly generated dendritic protrusions. Our data support a model in which astrocytic protrusive activity in development acts as a key local regulator for stabilization of individual dendritic protrusions and subsequent maturation into spines.