Experimental Manipulation of Pectin Architecture in the Cell Wall of the Unicellular Charophyte, Penium Margaritaceum
Experimental Manipulation of Pectin Architecture in the Cell Wall of the Unicellular Charophyte, Penium Margaritaceum
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DOI：
10.3389/fpls.2020.01032
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发表时间：
2020-07
影响因子：
5.6
通讯作者：
Kattia Palacio-López;Li Sun;Reagan Reed;E. Kang;Iben Sørensen;J. Rose;D. Domozych
中科院分区：
文献类型：
--
作者：
Kattia Palacio-López;Li Sun;Reagan Reed;E. Kang;Iben Sørensen;J. Rose;D. Domozych
果胶是植物初生细胞壁的主要成分之一。这些聚合物在细胞扩张、细胞间粘附和对生物应激的反应中发挥着关键作用。我们对单细胞链霉菌 Penium margaritaceum 的果胶结构进行了全面筛选。 Penium 拥有独特的细胞壁，其外层由富含果胶的纤维和突起组成的晶格组成。在这项研究中，细胞接受了各种物理、化学和酶处理，这些处理直接影响细胞壁，尤其是果胶晶格。使用场发射扫描电子显微镜、共焦激光扫描显微镜和透射电子显微镜对果胶晶格扰动进行的相关分析表明，果胶晶格微结构具有高度敏感性和可塑性。
Pectins represent one of the main components of the plant primary cell wall. These polymers have critical roles in cell expansion, cell-cell adhesion and response to biotic stress. We present a comprehensive screening of pectin architecture of the unicellular streptophyte, Penium margaritaceum. Penium possesses a distinct cell wall whose outer layer consists of a lattice of pectin-rich fibers and projections. In this study, cells were exposed to a variety of physical, chemical and enzymatic treatments that directly affect the cell wall, especially the pectin lattice. Correlative analyses of pectin lattice perturbation using field emission scanning electron microscopy, confocal laser scanning microscopy, and transmission electron microscopy demonstrate that pectin lattice microarchitecture is both highly sensitive and malleable.