Alpha-helix formation in melittin and beta-lactoglobulin A induced by fluorinated dialcohols.
Alpha-helix formation in melittin and beta-lactoglobulin A induced by fluorinated dialcohols.
复制标题
氟化二醇诱导蜂毒肽和 β-乳球蛋白 A 中的 α-螺旋形成。
DOI：
10.1021/jp056124l
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Baldwin,MelindaC
中科院分区：
文献类型：
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作者：
Schuh,MerlynD;Baldwin,MelindaC
广泛研究氟化醇对蛋白质构象的影响，特别是α-螺旋形成的诱导作用，由于其广泛的应用而具有重要意义。圆二色性（CD）显示氟化二醇2，2，3，3-四氟-1，4-丁二醇（TFBD）、2，2，3，3，4，4-六氟-1，6-戊二醇（HFPD）和2，2，3，3，4，4，5，5-八氟-1，6-己二醇（OFHD）对β-乳球蛋白A和蜂毒肽中螺旋诱导的增强作用以TFBD < HFPD < OFHD的顺序增加。对于氟化二醇和一元醇，发现螺旋诱导的有效性随着每个醇分子氟原子数量的增加而呈指数级增加，并且发现OFHD比任何先前报道的氟化醇更有效。标准胶束的形成被排除作为增强的螺旋诱导的氟化二醇的原因。蜂毒肽的荧光中的可忽略的红边激发位移表明，色氨酸发色团周围的氟化二醇/水溶剂壳比层状囊泡中的分子更不固定。
Extensive study of the effect of fluorinated alcohols on protein conformations, notably the induction of α-helix formation, is important because of its wide range of applications. Circular dichroism (CD) was used to show that the enhancement of helix induction in β-lactoglobulin A and melittin by the fluorinated diols 2,2,3,3-tetrafluoro-1,4-butanediol (TFBD), 2,2,3,3,4,4-hexafluoro-1,6-pentanediol (HFPD), and 2,2,3,3,4,4,5,5-octafluoro-1,6-hexanediol (OFHD) increases in the order TFBD < HFPD < OFHD. For fluorinated diols and monoalcohols the effectiveness of helix induction was found to increase exponentially with increasing number of fluorine atoms per alcohol molecule, and OFHD was found to be more effective than any previously reported fluorinated alcohol. Formation of standard micelles was ruled out as the cause of the enhanced helix induction by the fluorinated diols. The negligible red-edge excitation shift in the fluorescence of melittin indicated that the fluorinated diol/water solvent shell surrounding the tryptophan chromophore is less immobilized than are molecules in a lamellar vesicle.